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A pre-embedding immunolabeling technique for basal lamina and extracellular matrix molecules

MM Martins-Green and KT Tokuyasu

Department of Zoology, University of California, Davis 95616.

We have developed a pre-embedding immunolabeling technique to identify basal lamina and extracellular matrix molecules in embryos at various stages of development. The technique works for both fluorescence optical microscopy (1-2.5-micron sections) and for transmission electron microscopy, and enables straigthforward correlation between the two. An additional advantage is the easy preparation of well- oriented serial sections, facilitating detailed studies of development.

Volume 36, Issue 4, pp. 453-458, 04/01/1988
Copyright © 1988 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1988