|
|
|
|
 |
|||
|
|||
Direct staining and visualization of endothelial monolayers cultured on synthetic polycarbonate filters
PG Phillips and MF Tsan
Research Service, Albany Veterans Administration Medical Center, New York 12208.
Endothelial and epithelial cells cultured on synthetic filter supports have been used to study permeability and transport under various experimental conditions. However, because of the non-transparent nature of these filters, morphological studies using light microscopy are not possible. Presently, investigators circumvent this problem by using cells cultured on glass coverslips, extrapolating morphological data from a system clearly different from that used for functional studies. We describe here a useful technique for direct staining and visualization of cells grown on polycarbonate filter supports, using fluorochrome probes and fluorescence microscopy. We have utilized acridine orange, rhodamine phalloidin, and an anti-vimentin monoclonal antibody to provide information about cell shape, monolayer configuration, and cytoskeletal protein distribution in cultured calf pulmonary artery endothelial cell monolayers. Comparison staining of coverslip and filter preparations revealed a number of clear differences in these parameters. This technique should enable investigators to perform the necessary studies to obtain direct correlations between functional and morphological data.
Volume 36,
Issue 5,
pp. 551-554,
05/01/1988
Copyright © 1988 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Sawafuji, A. Ishizaka, M. Kohno, H. Koh, S. Tasaka, Y. Ishii, and K. Kobayashi Role of Rho-kinase in reexpansion pulmonary edema in rabbits Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L946 - L953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tasaka, H. Koh, W. Yamada, M. Shimizu, Y. Ogawa, N. Hasegawa, K. Yamaguchi, Y. Ishii, S. E. Richer, C. M. Doerschuk, et al. Attenuation of Endotoxin-Induced Acute Lung Injury by the Rho-Associated Kinase Inhibitor, Y-27632 Am. J. Respir. Cell Mol. Biol., June 1, 2005; 32(6): 504 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Phillips, L. M. Birnby, A. Narendran, and W. L. Milonovich Nitric oxide modulates capillary formation at the endothelial cell-tumor cell interface Am J Physiol Lung Cell Mol Physiol, July 1, 2001; 281(1): L278 - L290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Li and R. J. Mrsny Oncogenic Raf-1 Disrupts Epithelial Tight Junctions via Downregulation of Occludin J. Cell Biol., February 21, 2000; 148(4): 791 - 800. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |