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Polarized expression of an apical membrane glycoprotein is established before functional tight junctions have developed in MCF-7 cells

ZZ Zou, OW Petersen and B van Deurs

Department of Anatomy, Panum Institute, University of Copenhagen, Denmark.

A milk-fat globule membrane antigen, designated MAM-6 and detected immunocytochemically by the monoclonal antibody 115D8, is expressed apically in confluent MCF-7 monolayer cultures. Immediately after preparation of a single-cell suspension, MAM-6 appears on the entire cell surface. However, polarized apical expression of MAM-6 is restored as early as 2-6 hr after plating of unpolarized cells, before functional tight junctions are established, as judged by freeze- fracture and ruthenium red permeability. Quantitative immunogold cytochemistry reveals that the apical:basal ratio of MAM-6 expression was about 17:1 after 6 hr. Tight junctions developed as late as 12-24 hr after plating. At this time the apical:basal MAM-6 ratio was about 30:1 (as compared to about 50:1 in control monolayers).

Volume 37, Issue 1, pp. 15-24, 01/01/1989
Copyright © 1989 by The Histochemical Society


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