|
|
|
|
 |
|||
|
|||
Distribution of immunoreactive Na+,K+-ATPase in gerbil cochlea
BA Schulte and JC Adams
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston 29425.
The distribution of Na+,K+-ATPase was mapped in cochleas of mature gerbils with normal hearing, using a specific and sensitive immunocytochemical method. Na+,K+-ATPase was abundant in the basolateral plasma membrane of marginal cells in the stria vascularis. Considerable levels of enzyme were also associated with the surfaces of spiral ganglion neurons and their central and peripheral processes. An unexpected finding was the detection of high levels of immunoreactive Na+,K+-ATPase in three different populations of cells lying in the inferior portion of the spiral ligament and at the medial and lateral border of the scala vestibuli just superior to the attachment of Reissner's membrane. Cells in these areas shared the morphological characteristics of cells specialized for active transport but appeared to be nonpolarized, suggesting a uniform distribution of Na+,K+-ATPase over their entire plasmalemma. The presence of these three distinct cell populations in the cochlea of several mammalian species suggests that they play an important role in cochlear function, perhaps that of regulating the cation content of perilymph. The absence of discrete concentrations of Na+,K+-ATPase-rich cells in the perilymphatic connective tissue of the bird cochlea and the mammalian vestibular system suggests further that these cells may be involved with generating and maintaining the high endolymphatic potential unique to the mammalian cochlea.
Volume 37,
Issue 2,
pp. 127-134,
02/01/1989
Copyright © 1989 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Lang, V. Vallon, M. Knipper, and P. Wangemann Functional significance of channels and transporters expressed in the inner ear and kidney Am J Physiol Cell Physiol, October 1, 2007; 293(4): C1187 - C1208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shibata, H. Hibino, K. Doi, T. Suzuki, Y. Hisa, and Y. Kurachi Gastric type H+,K+-ATPase in the cochlear lateral wall is critically involved in formation of the endocochlear potential Am J Physiol Cell Physiol, November 1, 2006; 291(5): C1038 - C1048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Hibino and Y. Kurachi Molecular and physiological bases of the k+ circulation in the Mammalian inner ear. Physiology, October 1, 2006; 21: 336 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Wangemann Supporting sensory transduction: cochlear fluid homeostasis and the endocochlear potential J. Physiol., October 1, 2006; 576(1): 11 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Knipper, C. Claussen, L. Ruttiger, U. Zimmermann, R. Lullmann-Rauch, E.-L. Eskelinen, J. Schroder, M. Schwake, and P. Saftig Deafness in LIMP2-deficient mice due to early loss of the potassium channel KCNQ1/KCNE1 in marginal cells of the stria vascularis J. Physiol., October 1, 2006; 576(1): 73 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. S. Smith and J. Trimarchi Noninvasive measurement of hydrogen and potassium ion flux from single cells and epithelial structures Am J Physiol Cell Physiol, January 1, 2001; 280(1): C1 - C11. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Koyama, S. S. Spicer, and B. A. Schulte Distribution of I{kappa}B Proteins in Gastric Mucosa and Other Organs of Mouse and Gerbil J. Histochem. Cytochem., February 1, 2000; 48(2): 191 - 200. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Flagella, L. L. Clarke, M. L. Miller, L. C. Erway, R. A. Giannella, A. Andringa, L. R. Gawenis, J. Kramer, J. J. Duffy, T. Doetschman, et al. Mice Lacking the Basolateral Na-K-2Cl Cotransporter Have Impaired Epithelial Chloride Secretion and Are Profoundly Deaf J. Biol. Chem., September 17, 1999; 274(38): 26946 - 26955. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Minowa, K. Ikeda, Y. Sugitani, T. Oshima, S. Nakai, Y. Katori, M. Suzuki, M. Furukawa, T. Kawase, Y. Zheng, et al. Altered Cochlear Fibrocytes in a Mouse Model of DFN3 Nonsyndromic Deafness Science, August 27, 1999; 285(5432): 1408 - 1411. [Abstract] [Full Text]
|
|
|
|
|
|
M. Koyama, S. S. Spicer, and B. A. Schulte Immunohistochemical Localization of Ca2+/Calmodulin-dependent Protein Kinase IV in Outer Hair Cells J. Histochem. Cytochem., January 1, 1999; 47(1): 7 - 12. [Abstract] [Full Text]
|
|
|
|
|
|
N. Sakaguchi, M. T. Henzl, I. Thalmann, R. Thalmann, and B. A. Schulte Oncomodulin Is Expressed Exclusively by Outer Hair Cells in the Organ of Corti J. Histochem. Cytochem., January 1, 1998; 46(1): 29 - 40. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Hibino, Y. Horio, A. Inanobe, K. Doi, M. Ito, M. Yamada, T. Gotow, Y. Uchiyama, M. Kawamura, T. Kubo, et al. An ATP-Dependent Inwardly Rectifying Potassium Channel, KAB-2 (Kir4.1), in Cochlear Stria Vascularis of Inner Ear: Its Specific Subcellular Localization and Correlation with the Formation of Endocochlear Potential J. Neurosci., June 15, 1997; 17(12): 4711 - 4721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Crouch, N. Sakaguchi, C. Lytle, and B. A. Schulte Immunohistochemical Localization of the Na-K-Cl Co-transporter (NKCC1) in the Gerbil Inner Ear J. Histochem. Cytochem., January 1, 1997; 45(6): 773 - 778. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |