|
|
|
|
 |
|||
|
|||
Estimation of collagen content of liver specimens. Variation among animals and among hepatic lobes in cirrhotic rats
M Gascon-Barre, PM Huet, J Belgiorno, V Plourde and PA Coulombe
Centre de Recherche Clinique Andre-Viallet, Hopital Saint-Luc, Montreal, Quebec, Canada.
We undertook a study to evaluate the correlation between morphometric evaluation and colorimetric determination of hepatic collagen content, and to analyze the variation among animals as well as among lobes of the same liver in hepatic collagen content after CCl4-induced micronodular cirrhosis. The results revealed a significant correlation (r = 0.9458; p less than 0.001) between the morphometric and colorimetric methods of collagen evaluation of liver specimens; both methods also significantly distinguished data obtained from controls and from cirrhotic rats (p less than 0.0005). After induction of micronodular cirrhosis by chronic CCl4 administration, a highly significant variation in hepatic collagen content was observed among animals (p less than 0.0001). By contrast, no significant difference in collagen content was observed (p less than 0.05) among hepatic lobes of a given animal. These results indicate that in this animal model of liver cirrhosis, interpretation of biochemical data would benefit by being related to the severity of the hepatic collagen infiltration of each animal. Our data also show that representative values for total hepatic collagen infiltration can be obtained from a single liver specimen; we suggest, however, that the specimen be taken from a major lobe of the liver and that a sufficiently large number of animals be used to avoid occasional sampling errors.
Volume 37,
Issue 3,
pp. 377-381,
03/01/1989
Copyright © 1989 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. S. Kang, H. Wanibuchi, K. Morimura, R. Puatanachokchai, E. I. Salim, A. Hagihara, S. Seki, and S. Fukushima Enhancement by Estradiol 3-Benzoate in Thioacetamide-Induced Liver Cirrhosis of Rats Toxicol. Sci., May 1, 2005; 85(1): 720 - 726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Kang, K. Morimura, E. I. Salim, H. Wanibuchi, S. Yamaguchi, and S. Fukushima Persistence of Liver Cirrhosis in Association with Proliferation of Nonparenchymal Cells and Altered Location of {alpha}-Smooth Muscle Actin-Positive Cells Toxicol Pathol, April 1, 2005; 33(3): 329 - 335. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. W. Kobs, N. E. Muvarak, J. C. Eickhoff, and N. C. Chesler Linked mechanical and biological aspects of remodeling in mouse pulmonary arteries with hypoxia-induced hypertension Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1209 - H1217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Hornbuckle, C. A. Everett, C. C. Martin, S. S. Gustavson, C. A. Svitek, J. K. Oeser, D. W. Neal, A. D. Cherrington, and R. M. O'Brien Selective stimulation of G-6-Pase catalytic subunit but not G-6-P transporter gene expression by glucagon in vivo and cAMP in situ Am J Physiol Endocrinol Metab, May 1, 2004; 286(5): E795 - E808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Gross, I. Reibstein, L. Weiss, S. Slavin, H. Dafni, M. Neeman, M. Pines, and A. Nagler Treatment with Halofuginone Results in Marked Growth Inhibition of a von Hippel-Lindau Pheochromocytoma in Vivo Clin. Cancer Res., September 1, 2003; 9(10): 3788 - 3793. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Reifen, T. Nur, K. Ghebermeskel, G. Zaiger, R. Urizky, and M. Pines Vitamin A Deficiency Exacerbates Inflammation in a Rat Model of Colitis through Activation of Nuclear Factor-{kappa}B and Collagen Formation J. Nutr., September 1, 2002; 132(9): 2743 - 2747. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Jankov, X. Luo, P. Demin, R. Aslam, V. Hannam, A. K. Tanswell, and C. R. Pace-Asciak Hepoxilin Analogs Inhibit Bleomycin-Induced Pulmonary Fibrosis in the Mouse J. Pharmacol. Exp. Ther., May 1, 2002; 301(2): 435 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ilan, I. Gotsman, M. Pines, R. Beinart, M. Zeira, M. Ohana, E. Rabbani, D. Engelhardt, and A. Nagler Induction of oral tolerance in splenocyte recipients toward pretransplant antigens ameliorates chronic graft versus host disease in a murine model Blood, June 1, 2000; 95(11): 3613 - 3619. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |