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Immunohistochemical localization of relaxin in human prostate

RZ Sokol, XS Wang, J Lechago, PD Johnston and RS Swerdloff

Harbor-UCLA Medical Center, UCLA School of Medicine, Torrance 90502.

We identified relaxin in human male prostate by use of an anti-human relaxin analogue polyclonal antibody and the avidin-biotin- immunoperoxidase method. The antibody was obtained by immunizing a rabbit with a synthetic human relaxin analogue which has 95% sequence homology with native human relaxin. Human prostate tissues incubated with the anti-human relaxin analogue exhibited positive immunostaining up to an antibody dilution of 1:3200. Inhibition of immunostaining with this antibody by excess relaxin analogue demonstrated specificity of the antibody. The exact role of relaxin in human male reproductive physiology remains to be fully elucidated.

Volume 37, Issue 8, pp. 1253-1255, 08/01/1989
Copyright © 1989 by The Histochemical Society


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