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Indoxyl-tetranitro blue tetrazolium method for detection of alkaline phosphatase in immunohistochemistry

M Brenan and ML Bath

Department of Immunology, John Curtin School of Medical Research, Australian National University, Canberra.

A sensitive method for detection of alkaline phosphatase in immunohistochemistry, using lymphoid cells, has been optimized. The conditions for staining are 0.23 mM 5-bromo-4-chloro-indoxyl phosphate, 0.55 mM tetranitro blue tetrazolium, 2.0 mM levamisole, 5.0 mM sodium azide, 10.0 mM magnesium chloride, and 0.15 mM 1-methoxyphenazine methosulfate dissolved in 100 mM Tris-HCl buffer, pH 9.5.

Volume 37, Issue 8, pp. 1299-1301, 08/01/1989
Copyright © 1989 by The Histochemical Society


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