|
|
|
|
 |
|||
|
|||
Immunoelectron microscopic localization of aromatase in human placenta and ovary using microwave fixation
H Naganuma, H Ohtani, N Harada and H Nagura
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
In this study we investigated the immunohistochemical localization of a unique aromatase, a single protein of 51,000 daltons, in the human placenta and ovary at light and electron microscopic levels. Microwave fixation was adopted for the immunoelectron microscopic study because it is an excellent method for preserving antigenicity and subcellular structures in frozen sections. Tissue samples from four immature human placentas, four full-term human placentas, and two human ovaries fixed in 10% formalin were examined by light microscopy. In addition, tissues from three full-term human placentas and one immature human placenta fixed in 4% paraformaldehyde were examined by electron microscopy. By light microscopy, immunoreactivity for this aromatase was located in the syncytiotrophoblast and a part of the cytotrophoblast of the placenta and in the lutein and granulosa cells of the ovary. Immunoelectron microscopy revealed that the aromatase antigen was localized on the surface of the microvilli, the lateral plasma membrane, and in the endoplasmic reticulum (ER) in the syncytiotrophoblast of the placenta. The positive immunoreactivity in the syncytiotrophoblast ER is consistent with previous results using antibodies for other types of aromatase, whereas the reactivity on the microvilli has not been previously described. The present report describes the fine localization of this unique aromatase in placental and ovarian tissues; its localization on the plasma membranes requires further physiological investigation.
Volume 38,
Issue 10,
pp. 1427-1432,
10/01/1990
Copyright © 1990 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Sipahutar, P. Sourdaine, S. Moslemi, B. Plainfosse, and G.-E. Seralini Immunolocalization of Aromatase in Stallion Leydig Cells and Seminiferous Tubules J. Histochem. Cytochem., March 1, 2003; 51(3): 311 - 318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Lange and J. Gartzke Microvillar cell surface as a natural defense system against xenobiotics: a new interpretation of multidrug resistance Am J Physiol Cell Physiol, August 1, 2001; 281(2): C369 - C385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Mitchell, K. Feyereisen, S. Bouret, D. Leroy, and J.-C. Beauvillain Microwave Strategy for Improving the Simultaneous Detection of Estrogen Receptor and Galanin Receptor mRNA in the Rat Hypothalamus J. Histochem. Cytochem., July 1, 2001; 49(7): 901 - 910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. K. Rangell and G.-A. Keller Application of Microwave Technology to the Processing and Immunolabeling of Plastic-embedded and Cryosections J. Histochem. Cytochem., August 1, 2000; 48(8): 1153 - 1160. [Abstract] [Full Text]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |