|
|
|
|
 |
|||
|
|||
Ultrastructural immunocytochemical localization of excitatory amino acids in the somatosensory system
S De Biasi and A Rustioni
Dipartimento di Fisiologia, Sezione di Istologia ed Anatomia Umana, Milano, Italy.
We studied the ultrastructure and the synaptic arrangement of glutamate- immunoreactive terminals in rats, in the superficial laminae of the spinal cord, the brainstem cuneate nucleus, and the thalamic ventroposterolateral nucleus, where a role for glutamate as neurotransmitter has been suggested by biochemical, physiological and pharmacological approaches. The antiserum employed was raised against glutaramate conjugated to keyhole limpet hemocyanin with glutaraldehyde, and was used for pre-embedding staining with an avidin- biotin-peroxidase method and for post-embedding staining with an immunogold procedure. Both methods yielded similar results, consisting of labeling of selected terminals in all the areas examined. Double immunogold labeling on the same thin section using antisera against gamma-amino-butyric acid (GABA) or substance P (SP), in combination with the anti-glutamate serum, showed that staining for glutamate and GABA was present in different terminals in all the regions examined; glutamate and SP were co-localized in a few terminals only in the superficial laminae of the spinal cord. By performing immunogold staining in combination with anterograde tracing, glutamate immunoreactivity could be localized in identified primary afferents to the dorsal spinal cord and cuneate nucleus, and in lemniscal afferents to the thalamus.
Volume 38,
Issue 12,
pp. 1745-1754,
12/01/1990
Copyright © 1990 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. E. Landisman and B. W. Connors VPM and PoM Nuclei of the Rat Somatosensory Thalamus: Intrinsic Neuronal Properties and Corticothalamic Feedback Cereb Cortex, December 1, 2007; 17(12): 2853 - 2865. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Miyata, H. Kashiwadani, M. Fukaya, T. Hayashi, D. Wu, T. Suzuki, M. Watanabe, and Y. Kawakami Role of Thalamic Phospholipase C{beta}4 Mediated by Metabotropic Glutamate Receptor Type 1 in Inflammatory Pain J. Neurosci., September 3, 2003; 23(22): 8098 - 8108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Aimar, L. Pasti, G. Carmignoto, and A. Merighi Nitric Oxide-Producing Islet Cells Modulate the Release of Sensory Neuropeptides in the Rat Substantia Gelatinosa J. Neurosci., December 15, 1998; 18(24): 10375 - 10388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Peruzzi, E. Bartlett, P. H. Smith, and D. L. Oliver A Monosynaptic GABAergic Input from the Inferior Colliculus to the Medial Geniculate Body in Rat J. Neurosci., May 15, 1997; 17(10): 3766 - 3777. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |