|
|
|
|
 |
|||
|
|||
Endocytosis in cultured rat alveolar type II cells: effect of lysosomotropic weak bases on the processes
M Kalina and R Socher
Department of Histology and Cell Biology, Sackler School of Medicine, Tel-Aviv University, Israel.
We investigated the uptake of Lucifer yellow and surfactant complexed with gold (S-G) by isolated alveolar Type II cells. The fluid phase marker Lucifer yellow did not reach lamellar bodies (LB) even after prolonged incubation time, whereas S-G was internalized and found in LB. Treatment of Type II cells with lysosomotropic weak bases (NH4Cl and chloroquine) resulted in dilation of endosomes, lysosomes, and LB. The effect of these agents on LB resulted in disappearance of their lamellar organization, as detected by polarized light and electron microscopy. After incubation in lysosomotropic agent-free medium, endocytosis of Lucifer yellow and S-G in treated cells was mainly directed towards large vacuoles resembling either multivesicular bodies (MVB) or lysosomes. The possible relationship between LB, MVB, and lysosomes in freshly isolated as well as cultured alveolar Type II cells is discussed.
Volume 39,
Issue 10,
pp. 1337-1348,
10/01/1991
Copyright © 1991 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Khan, M. S. Bijker, J. J. Weterings, H. J. Tanke, G. J. Adema, T. van Hall, J. W. Drijfhout, C. J. M. Melief, H. S. Overkleeft, G. A. van der Marel, et al. Distinct Uptake Mechanisms but Similar Intracellular Processing of Two Different Toll-like Receptor Ligand-Peptide Conjugates in Dendritic Cells J. Biol. Chem., July 20, 2007; 282(29): 21145 - 21159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Werlinder, M. Backlund, A. Zhukov, and M. Ingelman-Sundberg Transcriptional and Post-Translational Regulation of CYP1A1 by Primaquine J. Pharmacol. Exp. Ther., April 1, 2001; 297(1): 206 - 214. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. A. Stevens, H. Wissel, S. Zastrow, D. Sieger, and K.-P. Zimmer Surfactant protein A and lipid are internalized via the coated-pit pathway by type II pneumocytes Am J Physiol Lung Cell Mol Physiol, January 1, 2001; 280(1): L141 - L151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Zen, K. Notarfrancesco, V. Oorschot, J. W. Slot, A. B. Fisher, and H. Shuman Generation and characterization of monoclonal antibodies to alveolar type II cell lamellar body membrane Am J Physiol Lung Cell Mol Physiol, July 1, 1998; 275(1): L172 - L183. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |