|
|
|
|
 |
|||
|
|||
Myofibrillar ATPase histochemistry of rat skeletal muscles: a "two- dimensional" quantitative approach
A Lind and D Kernell
Department of Neurophysiology, University of Amsterdam, The Netherlands.
In histochemical investigations of skeletal muscle, the fibers are commonly classified into three types according to their staining for myofibrillar ATPase (mATPase). In serial sections of skeletal muscles from normal Wistar rats, we compared two common staining methods for mATPase: (a) an ac-ATPase technique, with pre-incubation at pH 4.7, and (b) a fixed alk-ATPase technique, using treatment with 5% paraformaldehyde followed by pre-incubation at pH 10.4. In addition, the same fibers were stained in subsequent serial sections for succinate dehydrogenase (SDH) activity. Staining intensities were objectively evaluated by microphotometric measurements of optical density. Combining both mATPase methods in consecutive serial sections ("two-dimensional approach") led to the identification of four distinct clusters of fibers: Types I, IIA, and two subgroups of Type IIB, as separated by their staining densities for fixed alk-ATPase (IIBd dark, IIBm moderate). The mean intensity of SDH staining per fiber type, as measured in the central core of the fibers, was ranked such that IIA greater than I greater than IIBd greater than IIBm. The analyzed muscles (tibialis anterior, biceps brachii) were markedly heterogeneous with respect to the topographic distribution of different fiber types. In comparison to other muscle portions, the regions containing Type I fibers ("red" portions) showed a higher IIBd vs IIBm ratio and more intense SDH staining for either subtype of the IIB fibers. The IIBd fibers probably correspond to the Type 2X fibers of Schiaffino et al.
Volume 39,
Issue 5,
pp. 589-597,
05/01/1991
Copyright © 1991 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. C. Ryu and B. C. Kim Comparison of histochemical characteristics in various pork groups categorized by postmortem metabolic rate and pork quality J Anim Sci, April 1, 2006; 84(4): 894 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. G. Prado, I. Makarenko, C. Andresen, M. Kruger, C. A. Opitz, and W. A. Linke Isoform Diversity of Giant Proteins in Relation to Passive and Active Contractile Properties of Rabbit Skeletal Muscles J. Gen. Physiol., October 31, 2005; 126(5): 461 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sugiura and K. Kanda Progress of Age-Related Changes in Properties of Motor Units in the Gastrocnemius Muscle of Rats J Neurophysiol, September 1, 2004; 92(3): 1357 - 1365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Berchtold, H. Brinkmeier, and M. Muntener Calcium Ion in Skeletal Muscle: Its Crucial Role for Muscle Function, Plasticity, and Disease Physiol Rev, July 1, 2000; 80(3): 1215 - 1265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H Funakoshi, N Belluardo, E Arenas, Y Yamamoto, A Casabona, H Persson, and C. Ibanez Muscle-derived neurotrophin-4 as an activity-dependent trophic signal for adult motor neurons Science, June 9, 1995; 268(5216): 1495 - 1499. [Abstract] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |