|
|
|
|
 |
|||
|
|||
Immunocytochemical demonstration of a pancreatic secretory protein of unknown function in human duodenum
FO Senegas-Balas, CG Figarella, MA Amouric, OM Guy-Crotte, CA Bertrand and DC Balas
Groupe de Recherche de Biologie et de Pathologie Digestive, INSERM U 151, CHU Rangueil, Toulouse, France.
We have previously isolated from human pancreatic juice a secretory glycoprotein of 19 KD (P19), devoid of known enzymatic activity. P19 gave by proteolysis a protein of 14 KD (P14), at first named protein X and also called pancreatic thread protein or pancreatic stone protein. Specific rabbit immunosera prepared against P19 and P14 were applied to localize these proteins in human small intestine. By comparison, antibodies directed against some human pancreatic enzymes (amylase, lipase, chymotrypsin, trypsinogen 1, trypsinogen 2, and trypsin 1) were also tested. Positive immunoreactivity was observed on Paneth cells with antisera directed against trypsinogens, trypsin 1, and P19-related proteins. In addition, antisera directed against P19-related proteins stained the columnar cells located in the crypts of Lieberkuhn. These original findings are a further indication of the resemblance between Paneth and pancreatic acinar cells but show that their functional analogy is only partial. On the other hand, the presence of P19-related proteins on non-mature columnar cells suggests that this differential distribution is a consequence of differentiation.
Volume 39,
Issue 7,
pp. 915-919,
07/01/1991
Copyright © 1991 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Elphick, S. Liddell, and Y. R. Mahida Impaired Luminal Processing of Human Defensin-5 in Crohn's Disease: Persistence in a Complex with Chymotrypsinogen and Trypsin Am. J. Pathol., March 1, 2008; 172(3): 702 - 713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Shinozaki, T Nakamura, M Iimura, Y Kato, B Iizuka, M Kobayashi, and N Hayashi Upregulation of Reg 1{alpha} and GW112 in the epithelium of inflamed colonic mucosa Gut, May 1, 2001; 48(5): 623 - 629. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. R. Bernard–Perrone, W. P. Renaud, O. M. Guy–Crotte, P. Bernard, C. G. Figarella, H. Okamoto, D. C. Balas, and F. O. Senegas–Balas Expression of REG Protein During Cell Growth and Differentiation of Two Human Colon Carcinoma Cell Lines J. Histochem. Cytochem., July 1, 1999; 47(7): 863 - 870. [Abstract] [Full Text]
|
|
|
|
|
|
J Carrere, O Guy-Crotte, E Gaia, and C Figarella Immunoreactive pancreatic Reg protein in sera from cystic fibrosis patients with and without pancreatic insufficiency Gut, April 1, 1999; 44(4): 545 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Bernard-Perrone, J. Carrere, W. Renaud, C. Moriscot, K. Thoreux, P. Bernard, A. Servin, D. Balas, and F. Senegas-Balas Pancreatic trypsinogen I expression during cell growth and differentiation of two human colon carcinoma cells Am J Physiol Gastrointest Liver Physiol, June 1, 1998; 274(6): G1077 - G1086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Rubio, T. Lacaze-Masmonteil, B. Chailley-Heu, A. Kahn, J. R. Bourbon, and R. Ducroc Pulmonary Surfactant Protein A (SP-A) Is Expressed by Epithelial Cells of Small and Large Intestine J. Biol. Chem., May 19, 1995; 270(20): 12162 - 12169. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |