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R-phycoerythrin as a fluorescent label for immunolocalization of bound atrazine residues

G Sohn and C Sautter

Lehrstuhl fur Botanik, Fakultat fur Landwirtschaft und Gartenbau, Technische Universitat Munchen, Freising, Federal Republic of Germany.

We established a highly sensitive immunofluorescence procedure for localizing bound atrazine in the aquatic macrophytes Elodea canadensis and E. densa. The technique included biotin-labeled anti-rabbit IgG as a first enhancement step and R-phycoerythrin (R-PE) coupled to streptavidin for fluorescent labeling as a second improvement on the procedure. A comparison with the conventional indirect immunofluorescence method confirmed the superior results of the R-PE approach. The use of atrazine-free plants (grown in charcoal-filtered water) and a variety of other controls excluded both contaminating atrazine and nonspecific incubation constituents as sources of tissue staining. Pre-incubations to block nonspecific binding sites proved to be unnecessary in this system. The highly sensitive procedure described here might be a useful tool for the localization of tissue-bound pesticides in general and possibly of other haptens as well.

Volume 39, Issue 7, pp. 921-926, 07/01/1991
Copyright © 1991 by The Histochemical Society


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