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TWO TYPES OF ACIDOPHILS IN THE ANTERIOR PITUITARY OF THE FROG AND THEIR RELATION TO THE PERIODIC ACID SCHIFF REACTION

ROBERT ORTMAN 1

1 Department of Zoology, University of California, Berkeley

Koneff's ('38) modification of Mallory-azan reveals the presence of azocarmine, orange G, aniline blue, and chromophobic cells in the pars distalis of the frog.

A strong periodic acid Schiff (PAS) reaction, apparently of carbohydrate-containing material, uniformly distributed throughout the cytoplasm, is found in the azocarmine cells and in the aniline blue cells. Certain of the orange G cells contained PAS-positive material confined to cytoplasmic vesicles. The PAS-positive material is probably not glycogen. It was not possible to distinguish between the various PAS-reactive sites with the cytochemical methods employed in this study.

Submitted on April 18, 1956


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