|
|
|
|
 |
|||
|
|||
Localization of immunoreactive tyrosine hydroxylase in the goldfish retina with pre-embedding immunolabeling with one-nanometer colloidal gold particles and gold toning
DW Marshak
Department of Neurobiology and Anatomy, University of Texas Medical School, Houston 77225.
The goal of this study was to develop an alternative to silver intensification for visualizing small colloidal gold particles by light and electron microscopy. The isolated goldfish retina was labeled with rabbit antiserum to tyrosine hydroxylase and 1-nm colloidal gold- conjugated goat anti-rabbit IgG. The gold particles were enlarged by toning with gold chloride, followed by reduction in oxalic acid. Dopaminergic interplexiform cells were clearly visible by light microscopy and, in lightly-fixed material treated with detergent, they were labeled in their entirety. Labeling was qualitatively similar, although less extensive, in material fixed and processed for electron microscopy. The labeled processes were apparent in ultra-thin sections viewed at low magnification, but the gold-toned particles were not so large that they obscured subcellular structures. The procedure apparently had no deleterious effects on the tissue, since the ultrastructural preservation was comparable to that seen with other pre- embedding immunolabeling methods. The technique was simple, reliable and, since the gold solutions were so dilute, relatively inexpensive.
Volume 40,
Issue 10,
pp. 1465-1470,
10/01/1992
Copyright © 1992 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. M. Robinson, T. Takizawa, and D. D. Vandré Enhanced Labeling Efficiency Using Ultrasmall Immunogold Probes: Immunocytochemistry J. Histochem. Cytochem., April 1, 2000; 48(4): 487 - 492. [Abstract] [Full Text]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |