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A new acrylic resin formulation: a useful tool for histological, ultrastructural, and immunocytochemical investigations
C Scala, G Cenacchi, C Ferrari, G Pasquinelli, P Preda and GC Manara
Istituto di Microscopia Elettronica Clinica, Policlinico S. Orsola, Universita di Bologna, Italy.
We describe a new formulation for a hydrophilic resin, mostly composed of glycol methacrylate and hydroxypropyl methacrylate and here referred to as bioacryl, that allows the performance of morphological and immunohistochemical investigations at both light and electron microscopic levels. Immunolocalizations performed on bioacryl-embedded tissues are characterized by high specificity with virtually absent background staining. Finally, the new resin yields satisfactory fine- structural preservation, resulting in ultrastructural images of better quality than those obtained with Lowicryl K4M.
Volume 40,
Issue 11,
pp. 1799-1804,
11/01/1992
Copyright © 1992 by The Histochemical Society
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