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Differential extractability of calcium and pectic substances in different wall regions of epicotyl cells in young flax plants

A Jauneau, C Morvan, F Lefebvre, M Demarty, C Ripoll and M Thellier

Laboratoire des Echanges Cellularies, URA CNRS 203, Faculte des Sciences et des Techniques de Rouen, Mont-Saint-Aignan, France.

We applied the simultaneous use of a subtractive method and two imaging techniques (secondary ion mass spectrometry and electron microscopy after PATAg staining) to correlate the distribution of Ca2+ to pectic substances in cell walls of young flax plants. The calcium images were compared with the structural electron microscopy images. This suggests that the linkage of the pectic substances within the wall is mainly by calcium bridges in the intercellular junctions of most types of cells under study (epidermis, subepidermis, fiber layer, and endodermis) and in the outer part (close to the cuticle) of the wall of the epidermal cells. In the primary walls of the various types of cells under study and in the inner part (close to the cytoplasm) of the wall of the epidermal cells, the linkage of the pectic substances would be mainly by covalent bonds. In the middle lamellae of the various cells, and in the intercellular junctions within the cortical parenchyma, both types of linkages apparently coexist. The mechanism of "ionic condensation" may provide an interpretation for the chemical status of the Ca2+ ions which are associated with the pectic components solubilized in boiling water, and which do not seem to contribute to the linkage of these components within the wall.

Volume 40, Issue 8, pp. 1183-1189, 08/01/1992
Copyright © 1992 by The Histochemical Society


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I. His, C. Andeme-Onzighi, C. Morvan, and A. Driouich
Microscopic Studies on Mature Flax Fibers Embedded in LR White: Immunogold Localization of Cell Wall Matrix Polysaccharides
J. Histochem. Cytochem., December 1, 2001; 49(12): 1525 - 1536.
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