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Histochemical studies of acid and alkaline phosphatases in rat tooth germs with undecalcified resin-embedded specimens
D Tenorio, JP Germain and FJ Hughes
Department of Oral Medicine and Periodontology, London Hospital Medical College, United Kingdom.
A novel technique for the histochemical demonstration of acid phosphatase (AcPase) and alkaline phosphatase (AkPase) in hard tissues has been proposed. Fresh, unfixed, undecalcified samples of rat tooth germs and surrounding structures were embedded in LR Gold resin at -20 degrees C. Sections of 2 microns were taken and subsequently processed for enzyme histochemistry. AkPase reaction product appeared as strong linear staining outlining cell boundaries and was present in the enamel organ, dental pulp, and osteoblast cells. Tartrate-resistant AcPase staining was seen exclusively in the osteoclasts of developing alveolar bone. Our results demonstrated that the use of unfixed, undecalcified LR Gold resin-embedded specimens for histochemistry is a novel technique which may be of value for certain studies when decalcification of specimens is undesirable. The technique appears to give good preservation of enzyme activity combined with the ability to prepare sections with excellent morphological detail.
Volume 40,
Issue 8,
pp. 1229-1233,
08/01/1992
Copyright © 1992 by The Histochemical Society
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