|
|
|
|
 |
|||
|
|||
Immunohistochemical localization of proteoglycans in human periodontium
L Hakkinen, O Oksala, T Salo, F Rahemtulla and H Larjava
Department of Oral Biology, University of Turku, Finland.
Proteoglycans (PGs) are extracellular and cell surface-associated macromolecules that regulate cell adhesion, cell growth, matrix formation, and bind growth factors. In this work we studied the distribution of core proteins of four PGs (decorin, biglycan, a large molecular weight PG, and CD44) in human gingiva and periodontal ligament by immunohistochemical staining of frozen tissue sections with specific antibodies. Decorin, a major PG of this tissue, was localized on collagen fiber bundles in the gingival and periodontal connective tissues. Staining for decorin was most intense at the subepithelial region. Biglycan was a minor PG component of the human periodontium, showing some accumulation in connective tissue under the oral epithelium. At the immunohistochemical level, biglycan appeared to form fine filament-like structures on extracellular matrix fibers. Localization of large molecular weight PG differed from that of decorin and biglycan. It was concentrated in deep connective tissue areas of the gingiva and in the periodontal ligament, and was only weakly present at the subepithelial region. CD44 was mainly concentrated in cell-cell contact areas of basal and spinous layers of oral epithelium. In the connective tissue of gingiva and periodontal ligament, CD44 was localized on fibroblast cell surfaces. Connective tissue area under the junctional epithelium contained relatively small amounts of PGs. The results indicate that different parts of human periodontium contain a typical variety of PGs, suggesting a specific function for each PG species in the location at which they accumulate.
Volume 41,
Issue 11,
pp. 1689-1699,
11/01/1993
Copyright © 1993 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Hakkinen and A. Csiszar Hereditary Gingival Fibromatosis: Characteristics and Novel Putative Pathogenic Mechanisms Journal of Dental Research, January 1, 2007; 86(1): 25 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yamada, Y. Ozawa, M. Tomoeda, R. Matoba, K. Matsubara, and S. Murakami Regulation of PLAP-1 Expression in Periodontal Ligament Cells Journal of Dental Research, May 1, 2006; 85(5): 447 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nakamura, S. Sone, I. Takahashi, I. Mizoguchi, S. Echigo, and Y. Sasano Expression of Versican and ADAMTS1, 4, and 5 During Bone Development in the Rat Mandible and Hind Limb J. Histochem. Cytochem., December 1, 2005; 53(12): 1553 - 1562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. M. Bhide, C. A. Laschinger, P. D. Arora, W. Lee, L. Hakkinen, H. Larjava, J. Sodek, and C. A. McCulloch Collagen Phagocytosis by Fibroblasts Is Regulated by Decorin J. Biol. Chem., June 17, 2005; 280(24): 23103 - 23113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Waddington and G. Embery Proteoglycans and Orthodontic Tooth Movement J. Orthod., December 1, 2001; 28(4): 281 - 290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Bartold From Histoalchemy to Molecular Marvels: A Sojourn through Periodontal Connective Tissue Research Journal of Dental Research, January 1, 2001; 80(1): 305 - 308. [PDF]
|
|
|
|
|
|
A. Shimazu, M.A.H. Bachchu, M. Morishita, M. Noshiro, Y. Kato, and Y. Iwamoto Expression of Syndecan-2, -4, and Fibroblast Growth Factor Receptor Type 1 in Human Periodontal Ligament Fibroblasts and Down-regulation of These Membrane Proteins during Maturation in Culture Journal of Dental Research, December 1, 1999; 78(12): 1791 - 1799. [Abstract] [PDF]
|
|
|
|
|
|
O. Oksala, K. Haapasalmi, L. Hakkinen, V.-J. Uitto, and H. Larjava Expression of Heparan Sulphate and Small Dermatan/Chondroitin Sulphate Proteoglycans in Chronically Inflamed Human Periodontium Journal of Dental Research, June 1, 1997; 76(6): 1250 - 1259. [Abstract] [PDF]
|
|
|
|
|
|
L. Hakkinen, J. Westermarck, V.-M. Kahari, and H. Larjava Human Granulation-tissue Fibroblasts Show Enhanced Proteoglycan Gene Expression and Altered Response to TGF-{beta}1 Journal of Dental Research, October 1, 1996; 75(10): 1767 - 1778. [Abstract] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |