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Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR)
LE Lindner
Department of Pathology and Laboratory Medicine, College of Medicine, Texas A&M University, College Station 77843-1114.
The silver-staining technique for nucleolar organizer regions (AgNOR) of Ploton et al., as popularized by Crocker, is being widely used for evaluation of nucleolar function, especially in neoplasia. It suffers from limited reliability, background staining, precipitates, and fading of the sections. Factors were identified that affect these problems. The oxidation-reduction level and gelatin used are particularly important. An improved procedure is presented which incorporates pre- reduction of the sections, selection of an optimal gelatin, and post- treatment of the sections to produce a permanent preparation. It is compatible with many fixatives and with other stains used before or after the silver stain.
Volume 41,
Issue 3,
pp. 439-445,
03/01/1993
Copyright © 1993 by The Histochemical Society
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