|
|
|
|
 |
|||
|
|||
Localization of MOC-1 cell surface antigen in small-cell lung carcinoma cell lines: an immunohistochemical and immunoelectron microscopic study
V Speirs, S Eich-Bender, CR Youngson and E Cutz
Department of Pathology, Hospital for Sick Children, Toronto, Ontario, Canada.
Expression of cell surface antigens of the neural cell adhesion molecule (N-CAM) class was recently shown to be shared by both fetal and neoplastic neuroendocrine cells, including those of the lung. We investigated the expression and localization of MOC-1 antigen on small- cell (neuroendocrine) lung carcinoma cell lines with immunohistochemical methods at the light (LM) and electron microscopy (EM) level and by Western blot. At LM level, using monoclonal antibody (MAb) MOC-1 with the ABC method and immunofluorescence, positive staining was observed on surfaces of cells from all tumor lines examined. Strongest immunostaining was found on cell surfaces of pulmonary small-cell carcinoma-derived cell line NCI-H69 with the majority of cells showing positive staining. An adherent variant of NCI- H69 cell line, H69V, exhibited positive staining in about 60% of cells, whereas only occasional cells of NCI-H727 cell line derived from pulmonary carcinoid tumor were positive for MOC-1 antigen. Western blot analysis confirmed these findings, showing a strong MOC-1-specific band in cell extracts of NCI-H69, with weaker band densities for H69V and NCI-H727. Immunoelectron microscopy (IEM) revealed that MOC-1 was not uniformly distributed on the outer surface of plasma membrane; immunogold particles appeared concentrated in areas of thick cell surface "fuzz" coating, surface microvilli, and in areas of cell-cell contact. In some cells, areas of plasma membrane invaginations and a few intracytoplasmic vesicles were also labeled, suggesting endocytosis. Surface labeling for SEM confirmed the finding of more dense labeling over the microvilli, cell membrane folds, and in areas of cell-cell contact. The cell lines derived from pulmonary neuroendocrine cell tumors can provide a useful model to study the role and function of neural adhesion molecules in pulmonary neoplasia and during lung development.
Volume 41,
Issue 9,
pp. 1303-1310,
09/01/1993
Copyright © 1993 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Yeger, J. Pan, X. W. Fu, C. Bear, and E. Cutz Expression of CFTR and Cl{-} conductances in cells of pulmonary neuroepithelial bodies Am J Physiol Lung Cell Mol Physiol, September 1, 2001; 281(3): L713 - L721. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Wang, C. Youngson, V. Wong, H. Yeger, M. C. Dinauer, E. V.-S. de Miera, B. Rudy, and E. Cutz NADPH-oxidase and a hydrogen peroxide-sensitive K+ channel may function as an oxygen sensor complex in airway chemoreceptors and small cell lung carcinoma cell lines PNAS, November 12, 1996; 93(23): 13182 - 13187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Carsberg, K. Myers, G. Evans, T. Allen, and P. Stern Metastasis-associated 5T4 oncofoetal antigen is concentrated at microvillus projections of the plasma membrane J. Cell Sci., January 8, 1995; 108(8): 2905 - 2916. [Abstract] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |