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An alternative method for preparation of Schiff-like reagent from osmium-ammine complex for selective staining of DNA on thin Lowicryl sections

VT Mikhaylova and DV Markov

Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.

Batches of osmium-ammine (OA) complex vary considerably in staining properties when used in a Feulgen-like reaction for selective staining of DNA-containing structures. An alternative procedure for preparation of Schiff-like reagent from OA complex is described. It is based on generation of H2SO3, respectively SO2, within the OA solution and ensures more favorable conditions for production of a Schiff-like stain than bubbling with SO2 does. The method is reproducible and yields high staining intensity. Data obtained suggest that the ability of OA complex to produce Feulgen-like staining is strongly influenced by variations in its chemical composition. Their unfavorable effect can be overcome by selecting suitable conditions for preparation of a Schiff- like reagent. Conditions for obtaining specific and sensitive Feulgen- like staining are determined.

Volume 42, Issue 12, pp. 1643-1649, 12/01/1994
Copyright © 1994 by The Histochemical Society


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