|
|
|
|
 |
|||
|
|||
The SA/rABC technique: a new ABC procedure for detection of antigens at increased sensitivity
IM Grumbach and RW Veh
Zentrum fur Molekulare Neurobiologie, Hamburg, Germany.
Since its introduction, the avidin-biotin-peroxidase (ABC) complex has become an invaluable detection system for a wide variety of bioanalytical applications. In these techniques, avidin and biotin- peroxidase are mixed at a pre-determined ratio so that the soluble ABC complex retains biotin binding sites. Consequently, the complex contains an excess of avidin over biotinylated peroxidase residues. On theoretical considerations, however, an ABC complex designed for maximal signal intensity must consist of an excess of peroxidase over avidin molecules. Therefore, ABC complexes with reversed molar ratios of biotinylated peroxidase to avidin (rABC complexes) were prepared and an intermediate streptavidin step was introduced to bind the rABC complexes to biotinylated IgG molecules. The signal generating power of this new streptavidin-rABC sequence proved superior to that of the conventional ABC technique in ELISA assays and in immunostaining of tissue sections.
Volume 43,
Issue 1,
pp. 31-37,
01/01/1995
Copyright © 1995 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  D. von Smolinski, M. Blessenohl, C. Neubauer, K. Kalies, and A. Gebert Validation of a Novel Ultra-Short Immunolabeling Method for High-Quality mRNA Preservation in Laser Microdissection and Real-Time Reverse Transcriptase-Polymerase Chain Reaction J. Mol. Diagn., May 1, 2006; 8(2): 246 - 253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Sowden, N. Ballatori, K. L. d. M. Jensen, L. H. Reed, and H. C. Smith The editosome for cytidine to uridine mRNA editing has a native complexity of 27S: identification of intracellular domains containing active and inactive editing factors J. Cell Sci., January 3, 2002; 115(5): 1027 - 1039. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Phelps and D. L. Hurley Pituitary Hormones as Neurotrophic Signals: Update on Hypothalamic Differentiation in Genetic Models of Altered Feedback Experimental Biology and Medicine, October 2, 1999; 222(1): 39 - 58. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Pelosi, F. Pasini, F. Pavanel, E. Bresaola, I. Schiavon, and A. Iannucci Effects of Different Immunolabeling Techniques on the Detection of Small-cell Lung Cancer Cells in Bone Marrow J. Histochem. Cytochem., August 1, 1999; 47(8): 1075 - 1088. [Abstract] [Full Text]
|
|
|
|
|
|
M. I. Romero, M. A. Romero, and G. M. Smith Visualization of Axonally Transported Horseradish Peroxidase Using Enhanced Immunocytochemical Detection: A Direct Comparison with the Tetramethylbenzidine Method J. Histochem. Cytochem., February 1, 1999; 47(2): 265 - 272. [Abstract] [Full Text]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |