|
|
|
|
 |
|||
|
|||
The gene encoding vitamin K-dependent anticoagulant protein S is expressed in multiple rabbit organs as demonstrated by northern blotting, in situ hybridization, and immunohistochemistry
X He, L Shen, A Bjartell and B Dahlback
Department of Clinical Chemistry, Lund University, Malmo General Hospital, Sweden.
Vitamin K-dependent protein S is an anticoagulant plasma protein that functions as a co-factor to activated protein C in the degradation of coagulation factors Va and VIIIa. We investigated the tissue/cellular distribution of protein S synthesis by Northern blotting, in situ hybridization, and immunohistochemistry. Northern blotting together with in situ hybridization, using specific oligodeoxynucleotide probes, demonstrated protein S mRNA in liver, lung, testis, epididymis, ovary, uterus, and brain. In the reproductive system, protein S mRNA was present in the cytoplasm of Leydig cells, interstitial cells of the ovary, epithelial cells of the epididymis, and in the endometrium, including endometrial mucous glandular membrane in the myometrium. Bronchial epithelial cells and alveolar macrophages were positive in the respiratory system. In the central nervous system, pyramidal neurons in the cerebral cortex and in the hippocampal region, and dentate fascia neurons gave strongly positive signals. Immunohistochemistry with monoclonal antibodies yielded a staining pattern that correlated well with results of in situ hybridization. In conclusion, results from Northern blotting, in situ hybridization, and immunohistochemistry suggested that rabbit protein S is expressed in several extrahepatic tissues. The presence of protein S transcripts in these fully differentiated cells suggests a cell type-specific gene expression which may be related to local anticoagulation or to other as yet unknown protein S functions.
Volume 43,
Issue 1,
pp. 85-96,
01/01/1995
Copyright © 1995 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Wajih, D. C. Sane, S. M. Hutson, and R. Wallin Engineering of a Recombinant Vitamin K-dependent {gamma}-Carboxylation System with Enhanced {gamma}-Carboxyglutamic Acid Forming Capacity: EVIDENCE FOR A FUNCTIONAL CXXC REDOX CENTER IN THE SYSTEM J. Biol. Chem., March 18, 2005; 280(11): 10540 - 10547. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Benzakour and C. Kanthou The anticoagulant factor, protein S, is produced by cultured human vascular smooth muscle cells and its expression is up-regulated by thrombin Blood, March 15, 2000; 95(6): 2008 - 2014. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yamamoto and D. J. Loskutoff Extrahepatic Expression and Regulation of Protein C in the Mouse Am. J. Pathol., August 1, 1998; 153(2): 547 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. T. Smiley, S. N. Boyer, M. J. Heeb, J. H. Griffin, and M. J. Grusby Protein S is inducible by interleukin 4 in T cells and inhibits lymphoid cell procoagulant activity PNAS, October 14, 1997; 94(21): 11484 - 11489. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |