|
|
|
|
 |
|||
|
|||
Image analysis and image processing as tools to measure initial rates of enzyme reactions in sections: distribution patterns of glutamate dehydrogenase activity in rat liver lobules
A Jonker, WJ Geerts, R Charles, WH Lamers and CJ Van Noorden
Department of Anatomy and Embryology, University of Amsterdam, Academic Medical Center, The Netherlands.
To analyze regional differences in the activity of glutamate dehydrogenase in rat liver in situ, we developed an image recording and processing system for monitoring the formation of a colored final reaction product in time. All absorbance measurements of test and control reactions in time in consecutive sections were used to fit the data to a quadratic curve, with the derivative at t = 0 representing the initial velocity of formazan formation. The images of sections incubated for test and control reactions were topographically matched with an affine transformation using the positions of vessels as fiducials. Specific enzyme activity was calculated by subtracting the coefficients representing the initial velocity at corresponding locations in the test and control reactions and appeared to be 8 and 4 mumoles glutamate converted per min per cm3 of tissue at 20 degrees C in pericentral and periportal zones of fasted female rats, respectively. Those values are in agreement with biochemical data. The ability to construct two-dimensional images of cellular distribution patterns of enzyme activity in liver lobules is particularly useful for the study of metabolic zonation in this organ.
Volume 43,
Issue 10,
pp. 1027-1034,
10/01/1995
Copyright © 1995 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Boren, A. Ramos-Montoya, K. S. Bosch, H. Vreeling, A. Jonker, J. J. Centelles, M. Cascante, and W. M. Frederiks In Situ Localization of Transketolase Activity in Epithelial Cells of Different Rat Tissues and Subcellularly in Liver Parenchymal Cells J. Histochem. Cytochem., February 1, 2006; 54(2): 191 - 199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Boonacker and C. J.F. Van Noorden Enzyme Cytochemical Techniques for Metabolic Mapping in Living Cells, with Special Reference to Proteolysis J. Histochem. Cytochem., December 1, 2001; 49(12): 1473 - 1486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Winzer, C. J.F. Van Noorden, and A. Kohler Quantitative Cytochemical Analysis of Glucose-6-phosphate Dehydrogenase Activity in Living Isolated Hepatocytes of European Flounder for Rapid Analysis of Xenobiotic Effects J. Histochem. Cytochem., August 1, 2001; 49(8): 1025 - 1032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Biagiotti, K. S. Bosch, P. Ninfali, W. M. Frederiks, and C. J.F. Van Noorden Posttranslational Regulation of Glucose-6-phosphate Dehydrogenase Activity in Tongue Epithelium J. Histochem. Cytochem., July 1, 2000; 48(7): 971 - 978. [Abstract] [Full Text]
|
|
|
|
|
|
Y. Nakae and P. J. Stoward Effects of Tissue Protectants on the Kinetics of Lactate Dehydrogenase in Cells J. Histochem. Cytochem., October 1, 1997; 45(10): 1417 - 1426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. C. Geerts, A. Jonker, L. Boon, A. J. Meijer, R. Charles, C. J. F. Van Noorden, and W. H. Lamers In Situ Measurement of Glutamate Concentrations in the Periportal, Intermediate, and Pericentral Zones of Rat Liver J. Histochem. Cytochem., September 1, 1997; 45(9): 1217 - 1230. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |