|
|
|
|
 |
|||
|
|||
Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies
JL Whiteland, SM Nicholls, C Shimeld, DL Easty, NA Williams and TJ Hill
Department of Ophthalmology, University of Bristol, United Kingdom.
We describe a method for immunohistochemical localization of T-cells, CD4+ T-cells, CD8+ T-cells, B-cells, activated lymphocytes, major histocompatibility complex (MHC) class II antigens, macrophages, dendritic cells, and granulocytes in rat and mouse tissue fixed in periodate-lysine-paraformaldehyde (PLP) and embedded in paraffin. Rat and mouse spleen and eyes were fixed in PLP for 18-24 hr, rapidly dehydrated, infiltrated under vacuum with paraffin at 54 degrees C, sectioned, and stained with appropriate monoclonal antibodies (MAbs). Sections of PLP-fixed, paraffin-embedded spleen were compared with acetone-fixed frozen spleen sections with respect to morphology and staining quality. Nine of 10 MAbs to rat antigens and eight of nine MAbs to mouse antigens stained paraffin sections equally or more intensely than frozen sections. The two MAbs that showed weaker staining still gave good staining on paraffin sections. Paraffin- embedded rat and mouse eyes were easier to section serially than frozen eyes, showed superior morphology, and individually stained cells were readily identified. Therefore, a combination of PLP fixation and low- temperature paraffin embedding permits detection of the major types of immune cell in rat and mouse tissues while maintaining good morphology, particularly in diseased, damaged, or delicate tissues.
Volume 43,
Issue 3,
pp. 313-320,
03/01/1995
Copyright © 1995 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. F. Shubitz, S. M. Dial, R. Perrill, R. Casement, and J. N. Galgiani Vaccine-Induced Cellular Immune Responses Differ from Innate Responses in Susceptible and Resistant Strains of Mice Infected with Coccidioides posadasii Infect. Immun., December 1, 2008; 76(12): 5553 - 5564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Randall and G. Pearse A Dual-label Technique for the Immunohistochemical Demonstration of T-Lymphocyte Subsets in Formalin-fixed, Paraffin-Embedded Rat Lymphoid Tissue Toxicol Pathol, October 1, 2008; 36(6): 795 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Z. Rocha, E. J. Folco, G. Sukhova, K. Shimizu, I. Gotsman, A. H. Vernon, and P. Libby Interferon-{gamma}, a Th1 Cytokine, Regulates Fat Inflammation: A Role for Adaptive Immunity in Obesity Circ. Res., August 29, 2008; 103(5): 467 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Crowley, C. W. Frey, S. K. Gould, R. Griffiths, P. Ruiz, J. L. Burchette, D. N. Howell, N. Makhanova, M. Yan, H.-S. Kim, et al. Stimulation of lymphocyte responses by angiotensin II promotes kidney injury in hypertension Am J Physiol Renal Physiol, August 1, 2008; 295(2): F515 - F524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Ward, C. R. Erexson, L. J. Faucette, J. F. Foley, C. Dijkstra, and G. Cattoretti Immunohistochemical Markers for the Rodent Immune System Toxicol Pathol, August 1, 2006; 34(5): 616 - 630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. von Smolinski, M. Blessenohl, C. Neubauer, K. Kalies, and A. Gebert Validation of a Novel Ultra-Short Immunolabeling Method for High-Quality mRNA Preservation in Laser Microdissection and Real-Time Reverse Transcriptase-Polymerase Chain Reaction J. Mol. Diagn., May 1, 2006; 8(2): 246 - 253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Banerjee, F C Figueiredo, D L Easty, A D Dick, and S M Nicholls Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats Br. J. Ophthalmol., December 1, 2003; 87(12): 1515 - 1522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. H. Stumpf, R. Case, C. Shimeld, D. L. Easty, and T. J. Hill Primary herpes simplex virus type 1 infection of the eye triggers similar immune responses in the cornea and the skin of the eyelids J. Gen. Virol., June 1, 2002; 83(7): 1579 - 1590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. O'REGAN, J. M. HAYDEN, S. BODY, L. LIAW, N. MULLIGAN, M. GOETSCHKES, and J. S. BERMAN Abnormal Pulmonary Granuloma Formation in Osteopontin-deficient Mice Am. J. Respir. Crit. Care Med., December 15, 2001; 164(12): 2243 - 2247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. P. VIO, S.-J. AN, C. CESPEDES, J. C. MCGIFF, and N. R. FERRERI Induction of Cyclooxygenase-2 in Thick Ascending Limb Cells by Adrenalectomy J. Am. Soc. Nephrol., April 1, 2001; 12(4): 649 - 658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mueller, K. Wacker, W. F. Hickey, E. B. Ringelstein, and R. Kiefer Co-Localization of Multiple Antigens and Specific DNA : A Novel Method Using Methyl Methacrylate-Embedded Semithin Serial Sections and Catalyzed Reporter Deposition Am. J. Pathol., December 1, 2000; 157(6): 1829 - 1838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. McKay, J. Lu, S. Jedrzkiewicz, W. Ho, and K. A. Sharkey Nitric Oxide Participates in the Recovery of Normal Jejunal Epithelial Ion Transport Following Exposure to the Superantigen, Staphylococcus aureus Enterotoxin B J. Immunol., October 15, 1999; 163(8): 4519 - 4526. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Shimeld, D. L. Easty, and T. J. Hill Reactivation of Herpes Simplex Virus Type 1 in the Mouse Trigeminal Ganglion: an In Vivo Study of Virus Antigen and Cytokines J. Virol., March 1, 1999; 73(3): 1767 - 1773. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. P. Vio, C. Cespedes, P. Gallardo, and J. L. Masferrer Renal Identification of Cyclooxygenase-2 in a Subset of Thick Ascending Limb Cells Hypertension, September 1, 1997; 30(3): 687 - 692. [Abstract] [Full Text]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |