|
|
|
|
 |
|||
|
|||
Localized distribution of alpha 9 integrin in the cornea and changes in expression during corneal epithelial cell differentiation
MA Stepp, L Zhu, D Sheppard and RL Cranfill
Department of Anatomy, George Washington University Medical School, Washington, DC 20037.
A recently characterized integrin alpha-chain, alpha 9, forms heterodimers with the integrin beta 1-chain and is present in the skin with a distribution similar to that of alpha 2 and alpha 3, other beta 1 integrins. To determine whether alpha 9 is expressed in the stratified squamous epithelium of the cornea, we used immunohistochemical techniques to compare the distribution of alpha 9 in the adult mouse cornea with that of alpha 3. Abundant alpha 9 was expressed in the lateral and basal membranes of the basal cells of the conjunctiva and corneal limbus, but very little alpha 9 was present in the basal cells of the central corneal epithelium. In contrast, alpha 3 was present in the membranes of basal cells of the conjunctiva, limbus, and central cornea. To determine when during postnatal maturation of the corneal epithelium alpha 9 becomes restricted to the limbus, we looked at the distribution of alpha 9 and alpha 3 in the developing mouse eye from birth to eyelid opening. At birth, the basal cells of the cornea and developing limbal region did not express alpha 9, but there was abundant alpha 9 expressed in suprabasal cells between the fused lids and in the basal cells of the skin and conjunctiva. In contrast, alpha 3, integrin was expressed uniformly in the basal cells across the surface of the conjunctiva, limbus, and cornea and was present only in the basal cells of the epithelium between the fused eyelids. In the central cornea, alpha 9 expression increased in basal cells up until Day 10 after birth. After Day 10, alpha 9 expression in the central cornea began to decrease; after the lids were open, alpha 9 expression in the central cornea became restricted to the limbus. In the basal and suprabasal cells between the fused eyelids expression of alpha 9 became increasingly restricted over time to the basal cells. Recent data suggest that alpha 9 beta 1 can interact with tenascin. Our dual labeling confocal microscopy studies indicate that localization of alpha 9 and tenascin are not coordinated in the developing mouse cornea. Many recent studies have shown an important role for beta 1 integrins in mediating epithelial cell differentiation in vitro; in vivo, changes in integrin expression have been found in wound healing, psoriasis, and in basal and squamous cell carcinomas.(ABSTRACT TRUNCATED AT 400 WORDS)
Volume 43,
Issue 4,
pp. 353-362,
04/01/1995
Copyright © 1995 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Pal-Ghosh, A. Pajoohesh-Ganji, M. Brown, and M. A. Stepp A Mouse Model for the Study of Recurrent Corneal Epithelial Erosions: {alpha}9{beta}1 Integrin Implicated in Progression of the Disease Invest. Ophthalmol. Vis. Sci., June 1, 2004; 45(6): 1775 - 1788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Chen, C. S. de Paiva, L. Luo, F. L. Kretzer, S. C. Pflugfelder, and D.-Q. Li Characterization of Putative Stem Cell Phenotype in Human Limbal Epithelia Stem Cells, May 1, 2004; 22(3): 355 - 366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-E. Gingras, K. Larouche, N. Larouche, S. Leclerc, C. Salesse, and S. L. Guerin Regulation of the Integrin Subunit {alpha}5 Gene Promoter by the Transcription Factors Sp1/Sp3 Is Influenced by the Cell Density in Rabbit Corneal Epithelial Cells Invest. Ophthalmol. Vis. Sci., September 1, 2003; 44(9): 3742 - 3755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. SHEPPARD Functions of Pulmonary Epithelial Integrins: From Development to Disease Physiol Rev, July 1, 2003; 83(3): 673 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Ljubimov, M. Saghizadeh, R. Pytela, D. Sheppard, and M. C. Kenney Increased Expression of Tenascin-C-binding Epithelial Integrins in Human Bullous Keratopathy Corneas J. Histochem. Cytochem., November 1, 2001; 49(11): 1341 - 1350. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Lu, P. S. Reinach, and W. W.-Y. Kao Corneal Epithelial Wound Healing Experimental Biology and Medicine, July 1, 2001; 226(7): 653 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Z. Huang, J. F. Wu, R. Ferrando, J. H. Lee, Y. L. Wang, R. V. Farese Jr., and D. Sheppard Fatal Bilateral Chylothorax in Mice Lacking the Integrin alpha 9beta 1 Mol. Cell. Biol., July 15, 2000; 20(14): 5208 - 5215. [Abstract] [Full Text]
|
|
|
|
|
|
L. Häkkinen, H. C. Hildebrand, A. Berndt, H. Kosmehl, and H. Larjava Immunolocalization of Tenascin-C, {alpha}9 Integrin Subunit, and {alpha}v{beta}6 Integrin During Wound Healing in Human Oral Mucosa J. Histochem. Cytochem., July 1, 2000; 48(7): 985 - 998. [Abstract] [Full Text]
|
|
|
|
|
|
D. D. S. Iglesia, P. H. Gala, T. Qiu, and M. A. Stepp Integrin Expression During Epithelial Migration and Restratification in the Tenascin-C-deficient Mouse Cornea J. Histochem. Cytochem., March 1, 2000; 48(3): 363 - 376. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. L. Smith, H.-K. Cheung, L. E. Ling, J. Chen, D. Sheppard, R. Pytela, and C. M. Giachelli Osteopontin N-terminal Domain Contains a Cryptic Adhesive Sequence Recognized by alpha 9beta 1 Integrin J. Biol. Chem., November 8, 1996; 271(45): 28485 - 28491. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |