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In situ hybridization analyses of Na, K-ATPase alpha-subunit expression during early larval development of Artemia franciscana

R Escalante, A Garcia-Saez and L Sastre

Instituto de Investigaciones Biomedicas del Consejo Superior de Investigaciones Cientificas, Madrid, Spain.

The spatial pattern of expression of the mRNA encoded by the Na,K- ATPase alpha-subunit cDNA clone pArATNa136 was determined by in situ hybridization of first, second, and third instar Artemia franciscana larvae. This mRNA was expressed at high levels in the salt gland, the antennal gland, and the end of the midgut, which are the three main osmoregulatory organs in Artemia at these stages of development. The pattern of expression was similar at the three stages of development analyzed, although the level of expression increased during development, especially in the salt and antennal glands. The expression of the mRNA coding for another Na, K-ATPase alpha-subunit isoform, the proposed alpha 2-isoform, was also determined and was shown to be limited to the salt gland. These results suggest that the clone pArATNa136 codes for the biochemically defined alpha 1-isoform of the Na,K-ATPase alpha-subunit and reinforce the importance of this isoform in osmoregulation at the three larval stages studied. The alpha 2- isoform may also be involved in osmoregulation during the first stages of larval development.

Volume 43, Issue 4, pp. 391-399, 04/01/1995
Copyright © 1995 by The Histochemical Society


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 Mol Biol EvolHome page
A. G. Saez, R. Escalante, and L. Sastre
High DNA Sequence Variability at the {alpha}1 Na/K-ATPase Locus of Artemia franciscana (Brine Shrimp): Polymorphism in a Gene for Salt-Resistance in a Salt-Resistant Organism
Mol. Biol. Evol., February 1, 2000; 17(2): 235 - 250.
[Abstract] [Full Text] [PDF]



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