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Journal of Histochemistry and Cytochemistry, Vol. 45, 1317-1328, Copyright © 1997 by The Histochemical Society, Inc.

ARTICLE

Different Concentrations of Mg++ Ions Affect Nuclear Matrix Protein Distribution During Thermal Stabilization of Isolated Nuclei

Luca M. Neria,b, Beat M. Riedererc, Aurelio Valmorib, S. Capitania, and Alberto M. Martellid
a Istituto di Anatomia Umana Normale, Università di Ferrara, Ferrara, Italy
b Istituto di Citomorfologia Normale e Patologica del C.N.R., c/o Istituto Rizzoli, Bologna, Italy
c Institut de Biologie Cellulaire et de Morphologie, Université de Lausanne, Lausanne, Switzerland
d Dipartimento di Morfologia Umana Normale, Università di Trieste, Trieste, Italy

Correspondence to: Luca M. Neri, Istituto di Anatomia Umana Normale, via Fossato di Mortara 66, 44100 Ferrara, Italy.

The nuclear matrix, a proteinaceous network believed to be a scaffolding structure determining higher-order organization of chromatin, is usually prepared from intact nuclei by a series of extraction steps. In most cell types investigated the nuclear matrix does not spontaneously resist these treatments but must be stabilized before the application of extracting agents. Incubation of isolated nuclei at 37C or 42C in buffers containing Mg++ has been widely employed as stabilizing agent. We have previously demonstrated that heat treatment induces changes in the distribution of three nuclear scaffold proteins in nuclei prepared in the absence of Mg++ ions. We studied whether different concentrations of Mg++ (2.0-5 mM) affect the spatial distribution of nuclear matrix proteins in nuclei isolated from K562 erythroleukemia cells and stabilized by heat at either 37C or 42C. Five proteins were studied, two of which were RNA metabolism-related proteins (a 105-kD component of splicing complexes and an RNP component), one a 126-kD constituent of a class of nuclear bodies, and two were components of the inner matrix network. The localization of proteins was determined by immunofluorescent staining and confocal scanning laser microscope. Mg++ induced significant changes of antigen distribution even at the lowest concentration employed, and these modifications were enhanced in parallel with increase in the concentration of the divalent cation. The different sensitivity to heat stabilization and Mg++ of these nuclear proteins might reflect a different degree of association with the nuclear scaffold and can be closely related to their functional or structural role. (J Histochem Cytochem 45:1317-1328, 1997)

Key Words: erythroleukemia cells, nuclear matrix proteins, Mg++ ions, heat stabilization, immunocytochemistry, confocal microscopy


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