Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Montagne, J.-J.
Right arrow Articles by Bulant, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Montagne, J.-J.
Right arrow Articles by Bulant, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 45, 1623-1628, Copyright © 1997 by The Histochemical Society, Inc.

ARTICLE

Thyrotropin-releasing Hormone Immunoreactivity in Rat Adrenal Tissue Is Localized in Mast Cells

Jean-Jacques Montagnea, Ali Ladrama, Dominique Grouselleb, Pierre Nicolasa, and Marc Bulanta
a Laboratoire de Bioactivation des Peptides, Institut Jacques Monod, Paris, France
b Unité de Dynamique des Systèmes Neuroendocriniens, INSERM U 159, Paris, France

Correspondence to: Marc Bulant, Laboratoire de Bioactivation des Peptides, Institut Jacques Monod, 2 place Jussieu, 75251 Paris Cedex 05, France.

Pro-thyrotropin-releasing hormone (pro-TRH) has been shown to be present throughout the central nervous system and in several peripheral tissues. In adrenals, TRH immunoreactivity has been reported but not characterized. We show here that two rat pro-TRH-derived peptides, TRH and prepro-TRH[160-169] (Ps4), were detected in extracts of rat adrenal glands by enzyme immunoassay. Endogenous TRH and Ps4 were purified by gel exclusion chromatography and reverse-phase HPLC. Structural identification of each peptide was achieved by chromatographic comparison with synthetic standards. By using the indirect immunofluorescence technique, TRH-immunoreactive cell bodies were found rather widely scattered outside the adrenal, in the brown adipose tissue in which the gland is embedded. These immunofluorescent cells have the typical appearance of mast cells and are metachromatic after histological staining with acidic Toluidine Blue. Our findings suggest that pro-TRH-derived peptides exist in rat mast cells. (J Histochem Cytochem 45:1623-1627, 1997)

Key Words: neuropeptide, mast cells, immunocytochemistry, HPLC


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 EndocrinologyHome page
J.-J. Montagne, A. Ladram, P. Nicolas, and M. Bulant
Cloning of Thyrotropin-Releasing Hormone Precursor and Receptor in Rat Thymus, Adrenal Gland, and Testis
Endocrinology, March 1, 1999; 140(3): 1054 - 1059.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1997