Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jecker, P.
Right arrow Articles by Westermann, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jecker, P.
Right arrow Articles by Westermann, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 45, 393-402, Copyright © 1997 by The Histochemical Society, Inc.

ARTICLE

Long-term Oral Application of 5-Bromo-2-Deoxyuridine Does Not Reliably Label Proliferating Immune Cells in the LEW Rat

Peter Jeckerb, Andrea Beulekea, Ingeborg Dressendörfera, Reinhard Pabsta, and Jürgen Westermanna
a Centre of Anatomy, Medical School of Hannover, Hannover, Germany
b Clinic for Ear, Nose and Throat Diseases and for Plastic Surgery, RWTH Aachen, Aachen, Germany

Correspondence to: Jürgen Westermann, Zentrum Anatomie 4120, Medizinische Hochschule Hannover, 30623 Hannover, Germany.

To study the lifespan of immune cell populations in the LEW rat, 5-bromo-2-deoxyuridine (BrdU) was administered in the drinking water. After 12 weeks, the epithelium of gut and skin was completely BrdU+. In contrast, thymus, bone marrow, and germinal centers of Peyer's patches contained only a few BrdU+ cells, although most should have been labeled during this time. The lack of labeling was due neither to obvious toxic effects of BrdU on these organs nor to insufficient detection of incorporated BrdU. Analysis of the kinetic pattern of the appearance of BrdU+ cells in bone marrow, blood, spleen, and lymph nodes over 12 weeks revealed that the dosage of BrdU initially was high enough to label the proliferating cells in the bone marrow, but then became too low, although the BrdU uptake of the rats was similar over the entire time. This indicates that in the LEW rat the metabolism of orally applied BrdU changes over time, leading to a reduction in the amount of BrdU available for incorporation into the DNA below a level necessary for labeling all proliferating cells. This effect appears to be species- and strain-dependent, and should be considered when the BrdU technique is used. (J Histochem Cytochem 45:393-401, 1997)

Key Words: proliferation, oral application, BrdU, long-term labeling, LEW rat, Balb/c mouse, thymus, bone marrow, blood, spleen, lymph nodes


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Toxicol SciHome page
A. Al Kholaifi, A. Amer, B. Jeffery, T. J. B. Gray, R. A. Roberts, and D. R. Bell
Species-Specific Kinetics and Zonation of Hepatic DNA Synthesis Induced by Ligands of PPAR{alpha}
Toxicol. Sci., July 1, 2008; 104(1): 74 - 85.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. Chen, S. Turner, E. Tsang, J. Stark, H. Turner, A. Mahsut, K. Keifer, M. Goldfinger, and M. K. Hellerstein
Measurement of pancreatic islet cell proliferation by heavy water labeling
Am J Physiol Endocrinol Metab, November 1, 2007; 293(5): E1459 - E1464.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Westermann, U. Bode, A. Sahle, U. Speck, N. Karin, E. B. Bell, K. Kalies, and A. Gebert
Naive, Effector, and Memory T Lymphocytes Efficiently Scan Dendritic Cells In Vivo: Contact Frequency in T Cell Zones of Secondary Lymphoid Organs Does Not Depend on LFA-1 Expression and Facilitates Survival of Effector T Cells
J. Immunol., March 1, 2005; 174(5): 2517 - 2524.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. J. Kim, S. Cheung, and M. K. Hellerstein
Isolation of nuclei from label-retaining cells and measurement of their turnover rates in rat colon
Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1464 - C1473.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
N. M. Milicevic, B. Luettig, C. Trautwein, T. Wustefeld, M. Mahler, P. Jecker, K. Wonigeit, and J. Westermann
Splenectomy of rats selectively reduces lymphocyte function-associated antigen 1 and intercellular adhesion molecule 1 expression on B-cell subsets in blood and lymph nodes
Blood, November 15, 2001; 98(10): 3035 - 3041.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
B. Luettig, M. Kaiser, U. Bode, E. B. Bell, S. M. Sparshott, M. Bette, and J. Westermann
Naive and Memory T Cells Migrate in Comparable Numbers through the Normal Rat Lung . Only Effector T Cells Accumulate and Proliferate in the Lamina Propria of the Bronchi
Am. J. Respir. Cell Mol. Biol., July 1, 2001; 25(1): 69 - 77.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Luettig, L. Pape, U. Bode, E. B. Bell, S. M. Sparshott, S. Wagner, and J. Westermann
Naive and Memory T Lymphocytes Migrate in Comparable Numbers Through Normal Rat Liver: Activated T Cells Accumulate in the Periportal Field
J. Immunol., October 15, 1999; 163(8): 4300 - 4307.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1997