Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fujimori, K. E.
Right arrow Articles by Tamamaki, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fujimori, K. E.
Right arrow Articles by Tamamaki, N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 45, 455-460, Copyright © 1997 by The Histochemical Society, Inc.

TECHNICAL NOTE

A Procedure for In Situ Hybridization Combined with Retrograde Labeling of Neurons: Application to the Study of Cell Adhesion Molecule Expression in DiI-labeled Rat Pyramidal Neurons

Kazuhiro E. Fujimoria, Rumiko Takaujia, Yoshihiro Yoshiharab,c, Atsushi Tamadab,d, Kensaku Morib,d, and Nobuaki Tamamakia
a Department of Anatomy, Fukui Medical School, Matsuoka, Fukui, 910-11 Japan
b Department of Neuroscience, Osaka Bioscience Institute, Suita, Osaka, Japan
c Department of Biochemistry, Osaka Medical College, Takatsuki, Osaka, Japan
d Laboratory for Neuronal Recognition Molecules, Frontier Research Program, RIKEN, Wako, Saitama, Japan

Correspondence to: Kazuhiro E. Fujimori, Dept. of Anatomy, Fukui Medical School, Matsuoka, Fukui 910-11, Japan.

We have devised a simple method that combines retrograde labeling of projecting neurons and in situ hybridization histochemistry to examine mRNA expression in the retrogradely labeled neurons. First, projecting neurons were retrogradely labeled in vivo by injection of the lipophilic neuronal tracer DiI. The fluorescence of the labeled neurons in the brain slices was photoconverted into stable DAB precipitate by green light illumination. The slices were cut into thinner sections and processed for detection of specific mRNA by in situ hybridization. Using this highly sensitive method, we demonstrate here that the corticospinal tract neurons in newborn rats express mRNA for the cell adhesion molecule L1. TAG-1 mRNA was not detected in these neurons. Therefore, the present method provides an important tool to study the molecular expression of projection neurons during the development of neuronal circuitry. (J Histochem Cytochem 45:455-459, 1997)

Key Words: double labeling, in situ hybridization, L1, photoconversion, pyramidal neuron, rat, retrograde labeling, TAG-1


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1997