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Journal of Histochemistry and Cytochemistry, Vol. 45, 461-466, Copyright © 1997 by The Histochemical Society, Inc.

TECHNICAL NOTE

Widefield Microscopy Images of Tissue Sections by Computer Imaging Techniques

Isamu Ikedaa, Kazunobu Urushiharab, and Tomomichi Onoc
a Department of Dermatology, Mitsui Omuta Hospital, Fukuoka Japan
b Research Laboratory of Photographic Engineering, Kumamoto Institute of Technology, Kumamoto Japan
c Department of Dermatology, Kumamoto University School of Medicine, Kumamoto Japan

Correspondence to: Isamu Ikeda, Dept. Dermatology, Mitsui Omuta Hospital, Tenryo 1-100, Omuta City, Fukuoka 836, Japan.

A fine photomicrograph covering one whole specimen is very useful in the study of skin histopathology. However, because it is almost impossible to take such a picture with a conventional photomicroscope, we attempted to make one with the aid of a computer. The large field was divided into small fields, which were individually recorded through a photomicroscope. The images were then digitized and processed with a computer to reconstruct the largefield image. A fine seamless image was reconstructed with this method. We can thus extend the field of a photomicroscope with the aid of computer imaging techniques, without impairing the quality. (J Histochem Cytochem 45:461-466, 1997)

Key Words: photomicrography, computer imaging


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