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Journal of Histochemistry and Cytochemistry, Vol. 45, 551-558, Copyright © 1997 by The Histochemical Society, Inc.

ARTICLE

Phosphatidylinositol Transfer Protein in Lung: Cellular and Subcellular Localization

Abdul-Manaf A. Ibrahima and Jane D. Funkhousera
a Department of Biochemistry and Molecular Biology, University of South Alabama, Mobile, Alabama

Correspondence to: Jane D. Funkhouser, Dept. of Biochemistry and Molecular Biology, Univ. of South Alabama, 307 University Blvd., Mobile, AL 36688.

Determination of the cellular distribution of phosphatidylinositol transfer protein in rat lung by immunocytochemistry revealed that the protein is more readily observed in the nonciliated bronchial epithelial cells (Clara cells) than in other lung cells. By light microscopy, the phosphatidylinositol transfer protein (PtdIns-TP) was localized to the dome-shaped apical region of Clara cells that were identified by staining with an antibody to Clara cell protein. Further investigation by electron microscopy revealed that the PtdIns-TP accumulated at the limiting membrane surrounding secretory granules and at the apical plasma membrane. This localization is compatible with the proposed roles for PtdIns-TP in formation of vesicles and exocytosis of secretory granules and, when considered in the context of the proposed role of PtdIns-TP in phosphatidylinositide metabolism, suggests that phosphatidylinositides may be involved in the mechanisms regulating Clara cell secretion. (J Histochem Cytochem 45:551-558, 1997)

Key Words: phosphatidylinositol transfer protein, phospholipid transfer protein, Clara cell, Clara cell protein, secretion


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