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Journal of Histochemistry and Cytochemistry, Vol. 45, 569-582, Copyright © 1997 by The Histochemical Society, Inc.

ARTICLE

The Distribution of Laminins and Fibronectins Is Modulated During Extravillous Trophoblastic Cell Differentiation and Decidual Cell Response to Invasion in the Human Placenta

Matti Korhonena,b and Ismo Virtanenb
a Children's Hospital, University Hospital, University of Helsinki, Helsinki, Finland
b Department of Anatomy, Institute of Biomedicine, University of Helsinki, Helsinki, Finland

Correspondence to: Matti Korhonen, Dept of Anatomy, Inst. of Biomedicine, PO Box 9, FIN-00014 University of Helsinki, Finland.

We studied the distribution of laminin (Ln) {alpha}1-{alpha}3, ß1-ß3, and {gamma}1 chains, and of the extradomain-A (EDA) and EDB and the oncofetal epitope of fibronectin (Onc-Fn) in extravillous trophoblastic cells and decidua in the human placenta by immunohistochemistry. We found that the transition from villous to extravillous trophoblast was accompanied by emergence of immunoreactivity for EDA-, EDB-, and Onc-Fn among the cells. Furthermore, whereas the villous trophoblastic basement membrane (BM) contains Ln {alpha}1, {alpha}2, ß1, ß2, and {gamma}1 chains, immunoreactivity for Ln {alpha}1, ß1, and {gamma}1, but not for Ln {alpha}2 and ß2 chains, was detected in association with extravillous trophoblastic cells. Interestingly, although immunoreactivity for the Ln {alpha}1, {alpha}2, ß1, ß2, and {gamma}1 chains was detected in all decidual cell BMs, EDB-Fn and Onc-Fn were detected only in decidua that had been invaded by the trophoblast. In summary, our results describe distinct changes in the distribution of Ln and Fn isoforms during the differentiation of villous trophoblast into extravillous trophoblastic cells. Furthermore, EDB- and Onc-Fn are preferentially found in decidua that has been invaded by the trophoblast, indicating that the deposition of these Fn isoforms reflects a decidual cell response to invasion. (J Histochem Cytochem 45:569-581, 1997)

Key Words: placenta, human, extravillous trophoblastic cells, decidual cells, laminin, fibronectin, immunohistochemistry, basement membrane, extracellular matrix


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