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Journal of Histochemistry and Cytochemistry, Vol. 45, 883-894, Copyright © 1997 by The Histochemical Society, Inc.

ARTICLE

PDGFR{alpha} Expression During Mouse Embryogenesis: Immunolocalization Analyzed by Whole-mount Immunohistostaining Using the Monoclonal Anti-mouse PDGFR{alpha} Antibody APA5

Nobuyuki Takakuraa, Hisahiro Yoshidaa, Yasunori Oguraa, Hiroshi Kataokaa, Satomi Nishikawaa, and Shin-Ichi Nishikawaa
a Department of Molecular Genetics, Faculty of Medicine, Kyoto University, Kyoto, Japan

Correspondence to: Nobuyuki Takakura, Dept. of Molecular Genetics, Faculty of Medicine, Kyoto University, Shogoin Kawaharacho 53, Sakyo-ku 606-01 Japan.

We investigated the cells that express platelet-derived growth factor receptor {alpha} (PDGFR{alpha}) during mouse embryogenesis. PDGFR{alpha} expression has been identified by in situ hybridization or immunohistochemistry using polyclonal antibodies on tissue sections. Because no immunostaining study using whole-mount specimens has been published to date, we established a new monoclonal antibody (MAb), APA5, for this purpose. Our results differed in that APA5 stained only the paraxial mesoderm, whereas other investigators concluded that most if not all mesodermal cells expressed PDGFR{alpha}. Moreover, we did not find PDGFR{alpha} expression in embryonic erythrocytes, which have been previously suggested to express PDGFR{alpha}. On the basis of our present results, we wish to revise the proposed PDGFR{alpha} expression as follows. At the pregastrulation stage, PDGFR{alpha} is expressed only in primitive endoderm, particularly that in the ectoplacental cone. On gastrulation, it is expressed at high levels in the paraxial mesoderm. This expression continues after its differentiation into the somite. Along with the differentiation and migration of the sclerotome, PDGFR{alpha}1 cells begin to become distributed throughout the embryonal mesenchyme. During organogenesis, particularly intense staining is detected in regions of epithelial and mesenchymal interaction, such as the tooth bud and bronchi. In addition to mesodermal derivatives, the developing lens, apical ectodermal ridge, glial precursor, cardiac valves, and choroid plexus express PDGFR{alpha}. Our results with whole-mount immunostaining show that PDGFR{alpha} is abundantly expressed and may play important roles during embryogenesis. (J Histochem Cytochem 45:883-893, 1997)

Key Words: PDGFR{alpha}, mesoderm, somite, embryogenesis, whole-mount immunohistochemistry, mouse


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