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Journal of Histochemistry and Cytochemistry, Vol. 45, 895-902, Copyright © 1997 by The Histochemical Society, Inc.

TECHNICAL NOTE

A Simple Enzyme Histochemical Method for the Simultaneous Demonstration of Acetylcholinesterase and Monoamine Oxidase in Fixed-Frozen Sections

Daniel D. Dunninga, John G. McHaffieb, and Barry E. Steinb
a Department of Anatomy and Neurobiology, University of California, Irvine, California
b Department of Neurobiology and Anatomy, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina

Correspondence to: John G. McHaffie, Dept. of Neurobiology and Anatomy, Bowman Gray School of Medicine, Wake Forest U., Winston-Salem, NC 27157-1010.

We describe an enzyme histochemical technique for the simultaneous demonstration of acetylcholinesterase (AChE) and monoamine oxidase (MAO) (Types A, B, or A+B) in fixed-frozen sections. Several regions in the mesencephalon and brainstem were examined for both somatic and neuropil labeling. The results obtained are equivalent or superior to those obtained using previous methods for the individual localization of these enzymes. The simultaneous visualization of AChE and MAO in the same section allows the relationship of the two enzymes to be easily assessed with brightfield microscopy. (J Histochem Cytochem 45:895-902, 1997)

Key Words: acetylcholinesterase, monoamine oxidase, histocytochemistry, double labeling, locus coeruleus, superior colliculus, dorsal raphe


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