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Journal of Histochemistry and Cytochemistry, Vol. 46, 101-108, Copyright © 1998 by The Histochemical Society, Inc.

ARTICLE

Immunocytochemical Localization of the Prohormone Convertases PC1 and PC2 in Rat Prolactin Cells

Laurent Muller1,a, Renée Picarta, Alain Barreta, Nabil G. Seidahb, and Claude Tougarda
a Groupe de Biologie de la Cellule Neuroendocrine, INSERM U36, Collège de France, Paris, France
b Biochemical Neuroendocrinology, Clinical Research Institute of Montréal, Montreal, Quebec, Canada

Correspondence to: Claude Tougard, Groupe de Biologie de la Cellule Neuroendocrine, INSERM U 36, Collège de France, 11 Place Marcelin Berthelot, 75231 Paris Cedex 05, France.

The prohormone convertases PC1 and PC2 are subtilisin-related endopeptidases that process prohormone and neuropeptide precursors. Using different ultrastructural immunocytochemical approaches, we have investigated their intracellular distribution in a neuroendocrine cell type that has not been examined thus far, the rat anterior pituitary lactotrope. These cells secrete mainly prolactin and also express the neuroendocrine-specific protein secretogranin II, which is considered a peptide precursor. Our study provides evidence for the expression of PC1 and PC2 in rat lactotropes and provides new information on their subcellular localization. Apart from their presence in the secretory granules, PC1 and PC2 displayed different major localization along the secretory pathway. PC1 immunoreactivity was concentrated in the Golgi apparatus, whereas PC2 immunoreactivity was prominent in the rough endoplasmic reticulum (RER). These observations provide morphological support for previous biochemical analysis of proPC1 and proPC2 post-translational processing, which has demonstrated that PC1 exits very rapidly from the RER, whereas PC2 is retained much longer in this compartment. (J Histochem Cytochem 46:101-108, 1998)

Key Words: prohormone convertases, PC1, PC2, rat anterior pituitary, lactotropes, electron microscope, immunoperoxidase, immunogold


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