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Journal of Histochemistry and Cytochemistry, Vol. 46, 119-126, Copyright © 1998 by The Histochemical Society, Inc.

ARTICLE

Integrin Expression in Developing Smooth Muscle Cells

Rong Wanga, Marvin H. Stromera, and Ted W. Huiatta
a Muscle Biology Group, Department of Animal Science and Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa

Correspondence to: Marvin H. Stromer, Dept. of Animal Science, 3116 Molecular Biology Bldg., Iowa State U., Ames, IA 50011-3260.

We studied the specific expression patterns and distributions of {alpha}1 and ß1 integrin subunits, the major cell adhesion receptors in smooth muscle, in developing smooth muscle cells from 16-, 18-, and 20-day embryonic gizzards and from 1- and 7-day post hatch chick gizzards by SDS-PAGE, immunoblotting, and immunoelectron microscopy. Antibodies raised against {alpha}1 and ß1 integrins isolated from avian gizzards were used as probes. Gels and blots showed that the amount of {alpha}1 and ß1 integrins increased as age increased, with major increases at 1 and 7 days post hatch. Image analysis of immunoelectron micrographs demonstrated that statistically significant labeling increases occurred between embryonic Days 16 and 18, between embryonic Day 20 and 1 day post hatch, and between 1 day and 7 days post hatch. Immunolabeling with both anti-{alpha}1 and anti-ß1 integrin was prominent at membrane-associated dense plaques (MADPs) and at filament anchoring regions at cell ends. This indicates that {alpha}1 and ß1 integrin expression coincides temporally with the intracellular proliferation and reorientation of myofilaments. The similarity in distribution patterns of {alpha}1 and ß1 integrins during development suggests that the two integrin subunits are synchronously expressed during development and do not appear sequentially. (J Histochem Cytochem 46:119-125, 1998)

Key Words: integrin, developing smooth muscle, immunoelectron microscopy, immunoblots


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