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Journal of Histochemistry and Cytochemistry, Vol. 46, 91-100, Copyright © 1998 by The Histochemical Society, Inc.

ARTICLE

Immunolocalization of Vacuolar-type H+-ATPase in Rat Submandibular Gland and Adaptive Changes Induced by Acid-Base Disturbances

Eleni Roussaa, Frank Thévenodb, Ivan Sabolicb,d, Carol M. Herak-Krambergerd, Wolfgang Nastainczykc, Rudolf Bocka, and Irene Schulz
a Anatomisches Institut, Medizinische Fakultät, Universität des Saarlandes, Homburg/Saar, Germany
b II Physiologisches Institut, Medizinische Fakultät, Universität des Saarlandes, Homburg/Saar, Germany
c Institut für Biochemie, Medizinische Fakultät, Universität des Saarlandes, Homburg/Saar, Germany
d Institute for Medical Research and Occupational Health, Zagreb, Croatia

Correspondence to: Eleni Roussa, Anatomisches Institut, Medi-zinische Fakultät, Universität des Saarlandes, 66421 Homburg/Saar, Germany.

Using antibodies against the 31-kD and 70-kD subunits of vacuolar type H+-ATPase (V-ATPase) and light microscopic immunocytochemistry, we have demonstrated the presence of this V-ATPase in rat submandibular gland. We have also investigated the adaptive changes of this transporter during acid-base disturbances such as acute and chronic metabolic acidosis or alkalosis. Our results show intracellularly distributed V-ATPase in striated, granular, and main excretory duct cells in controls, but no V-ATPase immunoreaction in acinar cells. Both acute and chronic metabolic acidosis caused a shift in V-ATPase away from diffuse distribution towards apical localization in striated and granular duct cells, suggesting that a V-ATPase could be involved in the regulation of acid-base homeostasis. In contrast, during acidosis the main excretory duct cells showed no changes in the V-ATPase distribution compared to controls. With acute and chronic metabolic alkalosis, no changes in the V-ATPase distribution occurred. (J Histochem Cytochem 46:91-100, 1998)

Key Words: salivary glands, proton pump, immunocytochemistry, ion transport


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