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Journal of Histochemistry and Cytochemistry, Vol. 46, 1097-1102, October 1998, Copyright © 1998, The Histochemical Society, Inc.

RAPID COMMUNICATION

Correlative Microscopy Using FluoroNanogold on Ultrathin Cryosections: Proof of Principle

Toshihiro Takizawaa, Kouki Suzukia, and John M. Robinsonb
a Department of Anatomy, Jichi Medical School, Tochigi, Japan
b Department of Cell Biology, Neurobiology, and Anatomy, Ohio State University, Columbus, Ohio

Correspondence to: Toshihiro Takizawa, Dept. of Anatomy, Jichi Medical School, 3311 Yakushiji, Minamikawachi-machi, Tochigi 329-0498, Japan..

We demonstrate a fluorescent ultrasmall immunogold probe, FluoroNanogold (FNG), to be a versatile reporter system for immunocytochemical labeling of ultrathin cryosections. FNG-labeled molecules in the same ultrathin cryosections can be resolved by two imaging techniques (i.e., fluorescence and electron microscopy). Lactoferrin, a marker protein for the specific granules in human neutrophils, was employed as the target for FNG immunolabeling. The spatial resolution of the fluorescence signal from FNG-labeled specific granules was compatible with that of silver-enhanced gold signal from the same granules in electron microscopy. Our results confirm that FNG can be used as a probe for high-resolution correlation between immunofluorescence and electron microscopy. (J Histochem Cytochem 46:1097–1102, 1998)

Key Words: FluoroNanogold, immunocytochemistry, correlative microscopy, fluorescence microscopy, electron microscopy, ultrathin cryosections, lactoferrin, human neutrophils


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