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Journal of Histochemistry and Cytochemistry, Vol. 46, 1103-1112, October 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Nuclear Detection of Cellular Retinoic Acid Binding Proteins I and II with New Antibodies

Marie-Pierre Gauba, Yves Lutza, Norbert B. Ghyselincka, Isabelle Scheuera, Veronique Pfistera, Pierre Chambona, and Cécile Rochette–Eglya
a Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP/Collège de France, France

Correspondence to: Cécile Rochette–Egly, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), BP 163, 67404 Illkirch Cedex, C.U. de Strasbourg, France..

Apart from the retinoic acid nuclear receptor family, there are two low molecular weight (15 kD) cellular retinoic acid binding proteins, named CRABPI and II. Mouse monoclonal and rabbit polyclonal antibodies were raised against these proteins by using as antigens either synthetic peptides corresponding to amino acid sequences unique to CRABPI or CRABPII, or purified CRABP proteins expressed in E. coli. Antibodies specific for mouse and/or human CRABPI and CRABPII were obtained and characterized by immunocytochemistry and immunoblotting. They allowed the detection not only of CRABPI but also of CRABPII in both nuclear and cytosolic extracts from transfected COS-1 cells, mouse embryos, and various cell lines. (J Histochem Cytochem 46:1103–1111, 1998)

Key Words: cellular retinoic acid binding proteins, antibodies, immunodetection, nuclear localization


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