Identification of N- and O-linked Oligosaccharides in the Human EpididymisMaría I. Arenasa, Juan F. Madridb, Fermín R. Bethencourta, Benito Frailea, and Ricardo Paniaguaaa Department of Cell Biology and Genetics, University of Alcalá, Alcalá de Henares, Madrid b Department of Cell Biology, University of the Basque Country, Vizcaya, Spain Correspondence to: Ricardo Paniagua, Dept. of Cell Biology and Genetics, U. of Alcalá, 28871 Alcalá de Henares (Madrid), Spain.. The oligosaccharide sequences of glycoconjugates in the human normal epididymis and the nature of linkages were studied with lectin histochemistry. The usual terminal sequences of oligosaccharide side chains in epithelial cell secretions were Neu5Ac2,3Galß1,3GalNAc; SO4Galß1,3GalNAc; and Galß1,4GlcNAc, and they were mainly found in O-linked glycoproteins. The lectin pattern of mitochondria-rich cells differed from that of principal cells. (J Histochem Cytochem 46:11851188, 1998) Key Words: glycoconjugates, lectin histochemistry, oligosaccharide side chains, epididymis
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