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Journal of Histochemistry and Cytochemistry, Vol. 46, 1193-1198, October 1998, Copyright © 1998, The Histochemical Society, Inc.

BRIEF REPORT

Intracellular Retention of the Corticotrophin-releasing Hormone (CRH) Precursor Within COS-7 Cells

Marcelo J. Peronea, Simon Windeatta, Ewan Morrisona, Andy Sheringa, Peter Tomaseca, Elizabeth Lintonb, Pedro R. Lowensteina, and Maria G. Castroa
a Molecular Medicine Unit, Department of Medicine, University of Manchester, Manchester, United Kingdom
b Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Maternity Hospital University of Oxford, Oxford, United Kingdom

Correspondence to: Maria G. Castro, Molecular Medicine Unit, Dept. of Medicine, U. of Manchester, Stopford Bldg., Rm. 1.302, Oxford Road, Manchester M139PT, UK. E-mail: mcastro@fs2.scg. man.ac.uk..

We investigated the intracellular localization of CRH in transiently transfected COS-7 cells expressing the full-length rat corticotropin-releasing hormone (CRH) precursor cDNA. CRH synthesized by transfected COS-7 cells is mainly stored intracellularly. In contrast, CHO-K1 cells expressing the same CRH precursor stored and released equal amounts of immunoreactive (IR)-CRH. Ultrastructural analysis revealed that CRH is stored in electron-dense aggregates in the RER of transiently transfected COS-7 cells and does not migrate into the Golgi apparatus. On the basis of the different intracellular localization, storage, and release of CRH in COS-7 and CHO-K1 cells, we hypothesize that the intracellular trafficking of CRH within the constitutive secretory pathway for protein secretion not only depends on its primary amino acid sequence but might also be influenced by intracellular conditions or factors. (J Histochem Cytochem 46:1193–1197, 1998)

Key Words: corticotropin-releasing, hormone, intracellular trafficking, constitutive secretory pathway, COS-7, CHO


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