Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peters, B.
Right arrow Articles by Peters, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peters, B.
Right arrow Articles by Peters, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 46, 1215-1222, November 1998, Copyright © 1998, The Histochemical Society, Inc.

RAPID COMMUNICATION

Specific Regulation of StAR Expression in the Rat Adrenal Zona Glomerulosa: an In Situ Hybridization Study

Barbara Petersa, Susanne Clausmeyera, Nicholas Obermüllerb, Alexander Woythb, Bettina Kränzlinb, Norbert Gretzb, and Jörg Petersa
a Department of Pharmacology, University of Heidelberg, Heidelberg, Germany
b Medical Research Center, University of Heidelberg, Mannheim, Germany

Correspondence to: Barbara Peters, Dept. of Pharmacology, Univ. of Heidelberg, Im Neuenheimer Feld 366, D-69120 Heidelberg, Germany..

Steroid acute regulatory protein (StAR) plays an essential role in steroidogenesis because it is responsible for the transfer of cholesterol from cellular stores to the inner mitochondrial membrane. We investigated the distribution and regulation of StAR expression in association with aldosterone production in the rat adrenal gland in vivo. Using nonradioactive in situ hybridization, we demonstrate that the outermost five to seven parenchymal cell layers express the StAR gene only weakly and inhomogeneously. The strongest expression is found in the zona fasciculata and zona reticularis. In addition, some cells in the adrenal medulla also stained positively. To differentiate between functionally active glomerulosa and inactive intermediate cells, we compared the expression pattern of StAR with that of aldosterone synthase. The expression of the latter is localized to two or three cell layers only, located immediately below the capsule. However, the cells of the intermedia are capable of expressing both genes prominently, as shown after stimulation with bilateral nephrectomy for 2 days. All zones of the adrenal cortex by then expressed StAR gene to the same extent. This was accompanied by a 50-fold elevated plasma aldosterone concentration. Our data demonstrate that the width of the aldosterone-producing zone can increase within a short period of time by recruiting hormonally inactive cells to steroidogenesis. (J Histochem Cytochem 46:1215–1221, 1998)

Key Words: rat adrenal, glomerulosa, intermediate zone, nephrectomy, angiotensin II, StAR, aldosterone synthase, in situ hybridization


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
L. J. Martin, N. Boucher, C. Brousseau, and J. J. Tremblay
The Orphan Nuclear Receptor NUR77 Regulates Hormone-Induced StAR Transcription in Leydig Cells through Cooperation with Ca2+/Calmodulin-Dependent Protein Kinase I
Mol. Endocrinol., September 1, 2008; 22(9): 2021 - 2037.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
C. H Brennan, A. Chittka, S. Barker, and G. P Vinson
Eph receptors and zonation in the rat adrenal cortex
J. Endocrinol., July 1, 2008; 198(1): 185 - 191.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
B. Peters, P. Teubner, S. Clausmeyer, T. Puschner, C. Maser-Gluth, H.-J. Wrede, B. Kranzlin, and J. Peters
StAR expression and the long-term aldosterone response to high-potassium diet in Wistar-Kyoto and spontaneously hypertensive rats
Am J Physiol Endocrinol Metab, January 1, 2007; 292(1): E16 - E23.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
A. SPAT and L. HUNYADY
Control of Aldosterone Secretion: A Model for Convergence in Cellular Signaling Pathways
Physiol Rev, April 1, 2004; 84(2): 489 - 539.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. Salzmann, M. Otis, H. Long, C. Roberge, N. Gallo-Payet, and C.-D. Walker
Inhibition of Steroidogenic Response to Adrenocorticotropin by Leptin: Implications for the Adrenal Response to Maternal Separation in Neonatal Rats
Endocrinology, April 1, 2004; 145(4): 1810 - 1822.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
B. Peters, S. Clausmeyer, P. Teubner, N. Obermüller, B. Kränzlin, N. Gretz, T. Inagami, and J. Peters
Changes of AT2 Receptor Levels in the Rat Adrenal Cortex and Medulla Induced by Bilateral Nephrectomy and Its Modulation by Circulating ANG II
J. Histochem. Cytochem., May 1, 2001; 49(5): 649 - 656.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
J.-G. Lehoux, D. B. Hales, A. Fleury, N. Brière, D. Martel, and L. Ducharme
The in Vivo Effects of Adrenocorticotropin and Sodium Restriction on the Formation of Different Species of Steroidogenic Acute Regulatory Protein in Rat Adrenal
Endocrinology, November 1, 1999; 140(11): 5154 - 5164.
[Abstract] [Full Text]

Home page
 Circ. Res.Home page
S. Clausmeyer, R. Sturzebecher, and J. Peters
An Alternative Transcript of the Rat Renin Gene Can Result in a Truncated Prorenin That Is Transported Into Adrenal Mitochondria
Circ. Res., February 19, 1999; 84(3): 337 - 344.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Kantachuvesiri, S. Fleming, J. Peters, B. Peters, G. Brooker, A. G. Lammie, I. McGrath, Y. Kotelevtsev, and J. J. Mullins
Controlled Hypertension, a Transgenic Toggle Switch Reveals Differential Mechanisms Underlying Vascular Disease
J. Biol. Chem., September 21, 2001; 276(39): 36727 - 36733.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1998