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Journal of Histochemistry and Cytochemistry, Vol. 46, 1311-1320, November 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Lectin–Gold Localization of Fucose Residues in Human Gastric Mucosa

Juan Francisco Madrida, Olga Leisa, Lucio Díaz–Floresb, Francisco José Sáeza, and Francisco Hernándezc
a Department of Cell Biology and Morphological Sciences, School of Medicine and Dentistry, University of the Basque Country, Leioa, Vizcaya, Spain
b Department of Anatomy, Histology and Pathology, School of Medicine, University of La Laguna, Canary Islands, Spain
c Department of Cell Biology, School of Medicine, University of Murcia, Murcia, Spain

Correspondence to: Juan Francisco Madrid, Dept. of Cell Biology and Morphological Sciences, School of Medicine and Dentistry, Univ. of the Basque Country, E-48940 Leioa (Vizcaya), Spain. E-mail: gcpmacuj@lg.ehu.es.

The oligosaccharides of the mucous gastric glycoproteins are involved in the protection of the gastric mucosa and are altered in different diseases. Therefore, it is important to know their composition in health, to better determine the alterations induced by the disease. Moreover, analysis of the molecular composition of the fundic gland cells has been previously used to obtain new insights into the origin of the different cell types. The aim of the present study was the localization in the subcellular structures of the fucose residues of the oligosaccharides in human fundic glands. For this, lectin cytochemical methods were used at the light and electron microscopic levels. They were combined with enzymatic and chemical treatments to characterize the nature of the oligosaccharide chains containing the fucose residues. The presence of this carbohydrate belonging to N- or O-linked oligosaccharides has been demonstrated in the secretory granules of the surface, gastric pit, mucous neck, and transitional cells of the fundic mucosa, and in the intracellular canaliculi and tubulovesicular system of the parietal cells. These fucose residues were added in the trans-Golgi regions to the elongating chains. Additional fucose linked to the innermmost N-acetylglucosamine of the N-linked oligosaccharides was found in the chief cells, being incorporated in the cis-Golgi. The findings in the transitional cells corroborate the origin of the chief cells from the mucous neck cells. (J Histochem Cytochem 46:1311–1320, 1998)

Key Words: lectins, fucose, glycoproteins, oligosaccharides, stomach, human, differentiation, electron microscopy


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