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Journal of Histochemistry and Cytochemistry, Vol. 46, 1385-1392, December 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Specificity of Antibodies to Nitric Oxide Synthase Isoforms in Human, Guinea Pig, Rat, and Mouse Tissues

Wilko Coersa, Wim Timensa, Claudia Kempingaa, Pieter A. Klok, and Han Moshageb
a Departments of Pathology, University Hospital Groningen, Groningen, The Netherlands
b Gastroenterology and Hepatology, University Hospital Groningen, Groningen, The Netherlands

Correspondence to: Wim Timens, Dept. of Pathology, University Hospital Groningen, PO Box 30.001, 9700 RB Groningen, The Netherlands..

Ten commercially available rabbit polyclonal anti-NOS antibodies were tested for their immunohistological applicability in normal human, guinea pig, rat, and mouse organs. Most antibodies reacted as expected and described in the literature with various tissues of the investigated species. Several antibodies did not react with the expected cell populations in a certain species, or reacted in previously unknown patterns. In addition, different antibodies to the same isoform rarely detected identical cell populations, even within one species. Most of these unexpected immunoreactivities were observed in bronchial epithelial, glomerular epithelial, and vascular smooth muscle cells. These unexpected results usually occurred when the antibodies were tested in other organs or species than that to which they were originally raised. We therefore strongly recommend the use of anti-NOS antibodies only after careful immunohistological and biochemical analysis of their reactivity in the organ and species to be studied. (J Histochem Cytochem 46:1385–1391, 1998)

Key Words: nitric oxide, NOS isoforms, immunohistology, expression, characterization


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