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Journal of Histochemistry and Cytochemistry, Vol. 46, 1411-1422, December 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Quantitative Immunolocalization of a P29 Protein (GRA7), a New Antigen of Toxoplasma gondii

Annie Bonhommea, Gregory T. Maineb, Adrien Beorchiaa, Henriette Burleta, Dominique Aubertc, Isabelle Villenac, Jeff Huntb, Linda Chovanb, Larry Howardb, Sue Brojanacb, Michael Sheub, John Tynerb, Michel Pluotd, and Jean-Michel Pinona,b,c
a Equipe 4 INSERM U.314 IFR 53, CHU Maison Blanche, Reims, France
b Abbott Laboratories, Abbott Park, Illinois
c Laboratoire de Parasitologie EA 2070 IFR 53, CHU Maison Blanche, Reims, France
d Laboratoire d'Anatomie-Pathologique, CHU Hôpital Robert Debré, Reims, France

Correspondence to: Annie Bonhomme, INSERM U.314, IFR53, CHU Maison Blanche, 45, rue Cognacq Jay, 51092 Reims Cédex, France..

Ultrastructural localization of a P29 protein of Toxoplasma gondii was examined on thin sections by an immunogold technique using a P29 antigen-specific monoclonal antibody (5-241-178). Immunolocalization of the P29 protein in extracellular tachyzoites demonstrated that this antigen was present in the dense granules. Thus, we have identified this P29 antigen as the seventh protein (GRA7) to be localized to the dense granules of T. gondii. P29 immunolocalization in intracellular tachyzoites demonstrated association of this antigen with the parasite membrane complex, tubular elements of the intravacuolar network, and with the parasitophorous vacuolar membrane. Our immunolabeling data suggest trafficking of the P29 (GRA7) antigen from the dense granule via the intravacuolar network to the parasitophorous vacuolar membrane on invasion of the tachyzoite into the host cell. (J Histochem Cytochem 46:1411–1421, 1998)

Key Words: Toxoplasma gondii, GRA7, dense granules, antigen, parasitophorous vacuolar membrane


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[Abstract] [Full Text]



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