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Journal of Histochemistry and Cytochemistry, Vol. 46, 165-176, Copyright © 1998 by The Histochemical Society, Inc.

ARTICLE

Immunoelectron Microscopic Characterization of Human Dermal Lymphatic Microvascular Endothelial Cells: Differential Expression of CD31, CD34, and Type IV Collagen with Lymphatic Endothelial Cells vs Blood Capillary Endothelial Cells in Normal Human Skin, Lymphangioma, and Hemangioma In Situ

Birthe Sautera, Dagmar Foedingera, Barbara Sterniczkya, Klaus Wolffa, and Klemens Rappersbergera
a Department of Dermatology, Division of General Dermatology, University of Vienna, Medical School, Vienna, Austria

Correspondence to: Klemens Rappersberger, Dept. of Dermatology, Div. of General Dermatology, Waehringer Guertel 18-20, A-1090 Vienna, Austria.

We performed a comparative investigation of the immunomorphological characteristics of lymphatic and blood microvascular endothelial cells in normal human skin, cutaneous lymphangiomas, and hemangiomas, employing a pre-embedding immunogold electron microscopic technique. We stained for cell membrane proteins that are commonly used for light microscopic characterization of blood endothelial cells. With blood microvascular endothelial cells, we observed uniform labeling of the luminal cell membranes with monoclonal antibodies (MAbs) JC70 (CD31), EN-4 (CD31), BMA120, PAL-E, and QBEND-10 (CD34), and strong staining of the vascular basal lamina for Type IV collagen under normal and pathological conditions. In contrast, lymphatic microvascular endothelial cells in normal human skin and in lymphangiomas displayed, in addition to a luminal labeling, pronounced expression of CD31 and CD34 along the abluminal cell membranes. Moreover, CD31 was preferentially detected within intercellular junctions. The expression of CD34 was mostly confined to abluminal endothelial microprocesses and was upregulated in lymphangiomas and hemangiomas. Type IV collagen partially formed the luminal lining of initial lymphatics and occasionally formed bridges over interendothelial gaps. Our findings suggest a function of transmigration protein CD31 in recruitment of dendritic cells into the lymphatic vasculature. CD34 labeling may indicate early endothelial cell sprouting. The distribution of Type IV collagen also supports its role as a signal for migration and tube formation for lymphatic endothelial cells. (J Histochem Cytochem 46:165—176, 1998)

Key Words: immunoelectron, microscopy, immunomorphology, human dermal microvascular, endothelial cells, lymphatic capillaries, lymphatic endothelial cells, CD31, CD34, Type IV collagen


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