Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Saarnio, J.
Right arrow Articles by Sly, W. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saarnio, J.
Right arrow Articles by Sly, W. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Journal of Histochemistry and Cytochemistry, Vol. 46, 497-504, April 1998, Copyright © 1998, The Histochemical Society, Inc.

ARTICLE

Immunohistochemistry of Carbonic Anhydrase Isozyme IX (MN/CA IX) in Human Gut Reveals Polarized Expression in the Epithelial Cells with the Highest Proliferative Capacity

Juha Saarnioa, Seppo Parkkilab,d,e, Anna-Kaisa Parkkilab,d, Abdul Waheedd, Matthew C. Caseyd, Xiao Yan Zhoud, Silvia Pastorekováb,d,e, Jaromir Pastoreke, Tuomo Karttunenc, Kari Haukipuroa, Matti I. Kairaluomaa, and William S. Slyd
a Departments of Surgery, University of Oulu, Oulu, Finland
b Anatomy, University of Oulu, Oulu, Finland
c Pathology, University of Oulu, Oulu, Finland
d Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, St Louis, Missouri
e Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic

Correspondence to: William S. Sly, Edward A. Doisy Dept. of Biochemistry and Molecular Biology, Saint Louis U. School of Medicine, 1402 S. Grand Blvd., St Louis, MO 63104.

MN/CA IX is a recently discovered member of the carbonic anhydrase (CA) gene family that has been identified in the plasma membranes of certain tumor and epithelial cells and found to promote cell proliferation when transfected into NIH3T3 cells. This study presents localization of MN/CA IX in human gut and compares its distribution to those of CA I, II, and IV, which are known to be expressed in the intestinal epithelium. The specificity of the monoclonal antibody for MN/CA IX was confirmed by Western blots and immunostaining of COS-7 cells transfected with MN/CA IX cDNA. Immunohistochemical stainings of human gut revealed prominent polarized staining for MN/CA IX in the basolateral surfaces of the enterocytes of duodenum and jejunum, the reaction being most intense in the crypts. A moderate reaction was also seen in the crypts of ileal mucosa, whereas the staining became generally weaker in the large intestine. The results indicate isozyme-specific regulation of MN/CA IX expression along the cranial–caudal axis of the human gut and place the protein at the sites of rapid cell proliferation. The unique localization of MN/CA IX on the basolateral surfaces of proliferating crypt enterocytes suggests that it might serve as a ligand or a receptor for another protein that regulates intercellular communication or cell proliferation. Furthermore, MN/CA IX has a completely conserved active site domain of CAs suggesting that it could also participate in carbon dioxide/bicarbonate homeostasis. (J Histochem Cytochem 46:497–504, 1998)

Key Words: carbonic anhydrase, gastrointestinal, gut, human, immunohistochemistry, intestine, plasma membrane


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
A. M. Niemela, P. Hynninen, J.-P. Mecklin, T. Kuopio, A. Kokko, L. Aaltonen, A.-K. Parkkila, S. Pastorekova, J. Pastorek, A. Waheed, et al.
Carbonic Anhydrase IX Is Highly Expressed in Hereditary Nonpolyposis Colorectal Cancer
Cancer Epidemiol. Biomarkers Prev., September 1, 2007; 16(9): 1760 - 1766.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. E. Morgan, S. Pastorekova, A. K. Stuart-Tilley, S. L. Alper, and J. R. Casey
Interactions of transmembrane carbonic anhydrase, CAIX, with bicarbonate transporters
Am J Physiol Cell Physiol, August 1, 2007; 293(2): C738 - C748.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. Mizumori, J. Meyerowitz, T. Takeuchi, S. Lim, P. Lee, C. T. Supuran, P. H. Guth, E. Engel, J. D. Kaunitz, and Y. Akiba
Epithelial carbonic anhydrases facilitate PCO2 and pH regulation in rat duodenal mucosa
J. Physiol., June 15, 2006; 573(3): 827 - 842.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
P. Pan, M. Leppilampi, S. Pastorekova, J. Pastorek, A. Waheed, W. S. Sly, and S. Parkkila
Carbonic anhydrase gene expression in CA II-deficient (Car2-/-) and CA IX-deficient (Car9-/-) mice
J. Physiol., March 1, 2006; 571(2): 319 - 327.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
J. A. Haapasalo, K. M. Nordfors, M. Hilvo, I. J. Rantala, Y. Soini, A.-K. Parkkila, S. Pastorekova, J. Pastorek, S. M. Parkkila, and H. K. Haapasalo
Expression of Carbonic Anhydrase IX in Astrocytic Tumors Predicts Poor Prognosis
Clin. Cancer Res., January 15, 2006; 12(2): 473 - 477.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Leppilampi, S. Parkkila, T. Karttunen, M. O. Gut, G. Gros, and M. Sjoblom
Carbonic anhydrase isozyme-II-deficient mice lack the duodenal bicarbonate secretory response to prostaglandin E2
PNAS, October 18, 2005; 102(42): 15247 - 15252.
[Abstract] [Full Text] [PDF]

Home page
 Neuro OncolHome page
M. A. Proescholdt, C. Mayer, M. Kubitza, T. Schubert, S.-Y. Liao, E. J. Stanbridge, S. Ivanov, E. H. Oldfield, A. Brawanski, and M. J. Merrill
Expression of hypoxia-inducible carbonic anhydrases in brain tumors
Neuro-oncol, October 1, 2005; 7(4): 465 - 475.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
J. M. Purkerson and G. J. Schwartz
Expression of membrane-associated carbonic anhydrase isoforms IV, IX, XII, and XIV in the rabbit: induction of CA IV and IX during maturation
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2005; 288(5): R1256 - R1263.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. Hilvo, M. Rafajova, S. Pastorekova, J. Pastorek, and S. Parkkila
Expression of Carbonic Anhydrase IX in Mouse Tissues
J. Histochem. Cytochem., October 1, 2004; 52(10): 1313 - 1322.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
S. K. Chia, C. C. Wykoff, P. H. Watson, C. Han, R. D. Leek, J. Pastorek, K. C. Gatter, P. Ratcliffe, and A. L. Harris
Prognostic Significance of a Novel Hypoxia-Regulated Marker, Carbonic Anhydrase IX, in Invasive Breast Carcinoma
J. Clin. Oncol., August 15, 2001; 19(16): 3660 - 3668.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
P. Karhumaa, K. Kaunisto, S. Parkkila, A. Waheed, S. Pastorekova, J. Pastorek, W. S. Sly, and H. Rajaniemi
Expression of the transmembrane carbonic anhydrases, CA IX and CA XII, in the human male excurrent ducts
Mol. Hum. Reprod., July 1, 2001; 7(7): 611 - 616.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
N. J. P. Beasley, C. C. Wykoff, P. H. Watson, R. Leek, H. Turley, K. Gatter, J. Pastorek, G. J. Cox, P. Ratcliffe, and A. L. Harris
Carbonic Anhydrase IX, an Endogenous Hypoxia Marker, Expression in Head and Neck Squamous Cell Carcinoma and its Relationship to Hypoxia, Necrosis, and Microvessel Density
Cancer Res., July 1, 2001; 61(13): 5262 - 5267.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. Ivanov, S.-Y. Liao, A. Ivanova, A. Danilkovitch-Miagkova, N. Tarasova, G. Weirich, M. J. Merrill, M. A. Proescholdt, E. H. Oldfield, J. Lee, et al.
Expression of Hypoxia-Inducible Cell-Surface Transmembrane Carbonic Anhydrases in Human Cancer
Am. J. Pathol., March 1, 2001; 158(3): 905 - 919.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
C. C. Wykoff, N. Beasley, P. H. Watson, L. Campo, S. K. Chia, R. English, J. Pastorek, W. S. Sly, P. Ratcliffe, and A. L. Harris
Expression of the Hypoxia-Inducible and Tumor-Associated Carbonic Anhydrases in Ductal Carcinoma in Situ of the Breast
Am. J. Pathol., March 1, 2001; 158(3): 1011 - 1019.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Ulmasov, A. Waheed, G. N. Shah, J. H. Grubb, W. S. Sly, C. Tu, and D. N. Silverman
Purification and kinetic analysis of recombinant CA XII, a membrane carbonic anhydrase overexpressed in certain cancers
PNAS, December 19, 2000; 97(26): 14212 - 14217.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
S. Parkkila, A.-K. Parkkila, J. Saarnio, J. Kivelä, T. J. Karttunen, K. Kaunisto, A. Waheed, W. S. Sly, O. Türeci, I. Virtanen, et al.
Expression of the Membrane-associated Carbonic Anhydrase Isozyme XII in the Human Kidney and Renal Tumors
J. Histochem. Cytochem., December 1, 2000; 48(12): 1601 - 1608.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. S. Knuppel-Ruppert, G. Gros, W. Harringer, and H.-P. Kubis
Immunochemical evidence for a unique GPI-anchored carbonic anhydrase isozyme in human cardiomyocytes
Am J Physiol Heart Circ Physiol, April 1, 2000; 278(4): H1335 - H1344.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A. Kivela, S. Parkkila, J. Saarnio, T. J. Karttunen, J. Kivela, A.-K. Parkkila, A. Waheed, W. S. Sly, J. H. Grubb, G. Shah, et al.
Expression of a Novel Transmembrane Carbonic Anhydrase Isozyme XII in Normal Human Gut and Colorectal Tumors
Am. J. Pathol., February 1, 2000; 156(2): 577 - 584.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
P. Karhumaa, S. Parkkila, O. Tureci, A. Waheed, J.H. Grubb, G. Shah, A.-K. Parkkila, K. Kaunisto, J. Tapanainen, W.S. Sly, et al.
Identification of carbonic anhydrase XII as the membrane isozyme expressed in the normal human endometrial epithelium
Mol. Hum. Reprod., January 1, 2000; 6(1): 68 - 74.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Waheed, S. Parkkila, J. Saarnio, R. E. Fleming, X. Y. Zhou, S. Tomatsu, R. S. Britton, B. R. Bacon, and W. S. Sly
Association of HFE protein with transferrin receptor in crypt enterocytes of human duodenum
PNAS, February 16, 1999; 96(4): 1579 - 1584.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. V. Ivanov, I. Kuzmin, M.-H. Wei, S. Pack, L. Geil, B. E. Johnson, E. J. Stanbridge, and M. I. Lerman
Down-regulation of transmembrane carbonic anhydrases in renal cell carcinoma cell lines by wild-type von Hippel-Lindau transgenes
PNAS, October 13, 1998; 95(21): 12596 - 12601.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A.-K. Parkkila, A. L. Scarim, S. Parkkila, A. Waheed, J. A. Corbett, and W. S. Sly
Expression of Carbonic Anhydrase V in Pancreatic Beta Cells Suggests Role for Mitochondrial Carbonic Anhydrase in Insulin Secretion
J. Biol. Chem., September 18, 1998; 273(38): 24620 - 24623.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
O. Tureci, U. Sahin, E. Vollmar, S. Siemer, E. Gottert, G. Seitz, A.-K. Parkkila, G. N. Shah, J. H. Grubb, M. Pfreundschuh, et al.
Human carbonic anhydrase XII: cDNA cloning, expression, and chromosomal localization of a carbonic anhydrase gene that is overexpressed in some renal cell cancers
PNAS, June 23, 1998; 95(13): 7608 - 7613.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1998